Mouse Interleukin 4 (IL-4) ELISA Kit | IL-4 elisa kit

Mouse Interleukin 4 (IL-4) ELISA Kit

Cat. Num. AAA261893

• Gene Names: IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Reactivity: Mouse
• Synonyms: Interleukin 4 (IL-4); Mouse Interleukin 4 (IL-4) ELISA Kit; IL-4 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 153

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-4 concentration
• Assay Type: Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-4 elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse IL-4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504669
• NCBI GeneID: 3565
• NCBI Accession #: NP_000580.1
• NCBI GenBank Nucleotide #: NM_000589.3
• Molecular Weight: 15,797 Da
• NCBI Official Full Name: interleukin-4 isoform 1
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• NCBI Protein Information: interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; interleukin 4 variant 2; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell cycle regulation; Motility/polarity/chemotaxis; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; positive regulation of isotype switching to IgG isotypes; negative regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; microglial cell activation; regulation of proton transport; female pregnancy; chemotaxis; positive regulation of activated T cell proliferation; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; connective tissue growth factor biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; negative regulation of chronic inflammatory response; regulation of immune response; T-helper 2 type immune response; regulation of phosphorylation; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; positive regulation of chemokine biosynthetic process; response to ethanol; positive regulation of T cell differentiation; innate immune response in mucosa; positive regulation of tyrosine phosphorylation of Stat5 protein; retina development in camera-type eye; B cell differentiation; response to cytokine stimulus; cellular defense response; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis

Disease: Stroke, Ischemic