Goat Interleukin 1 Alpha (IL1a) ELISA Kit | IL1a elisa kit

Goat Interleukin 1 Alpha (IL1a) High Sensitive ELISA Kit

Cat. Num. AAA2707972

• Reactivity: Goat
• Applications: ELISA
• Synonyms: Interleukin 1 Alpha (IL1a); Goat Interleukin 1 Alpha (IL1a) High Sensitive ELISA Kit; Interleukin 1 Alpha; IL1-A; IL-1alpha; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1; IL1a elisa kit

• Reactivity: Goat
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Interleukin 1 Alpha (IL1a).; No significant cross-reactivity or interference between High Sensitive Interleukin 1 Alpha (IL1a) and analogues was observed.
• Sequence Length: 268

• Applicable Applications for IL1a elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 0.781-50pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.32pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 1 Alpha (IL1a) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 1 Alpha (IL1a) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 1 Alpha (IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 1 Alpha (IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 1 Alpha (IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Interleukin 1 Alpha (IL1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 6016355
• NCBI GeneID: 100861289
• UniProt Accession #: P79161
• NCBI Official Full Name: Interleukin-1 alpha
• NCBI Official Symbol: IL1A
• NCBI Protein Information: interleukin-1 alpha; LOW QUALITY PROTEIN: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL-1 alpha

Uniprot Description

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells ().