Mouse Integrin Beta 6 (ITGb6) ELISA Kit | ITGb6 elisa kit

Mouse Integrin Beta 6 (ITGb6) ELISA Kit

Cat. Num. AAA2704173

• Gene Names: ITGB6; AI1H
• Reactivity: Mouse
• Synonyms: Integrin Beta 6 (ITGb6); Mouse Integrin Beta 6 (ITGb6) ELISA Kit; ITG-B6; ITGb6 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Beta 6 (ITGb6).; No significant cross-reactivity or interference between Integrin Beta 6 (ITGb6) and analogues was observed.
• Sequence Length: 715

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 6 (ITGb6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 6 (ITGb6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGb6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 6 (ITGb6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 6 (ITGb6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 6 (ITGb6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 6 (ITGb6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 538917858
• NCBI GeneID: 3694
• NCBI Accession #: NP_001269318.1
• NCBI GenBank Nucleotide #: NM_001282389.1
• Molecular Weight: 74,958 Da
• NCBI Official Full Name: integrin beta-6 isoform e
• NCBI Official Synonym Full Names: integrin subunit beta 6
• NCBI Official Symbol: ITGB6
• NCBI Official Synonym Symbols: AI1H
• NCBI Protein Information: integrin beta-6
• UniProt Protein Name: Integrin beta-6
• Protein Family: Integrin
• UniProt Gene Name: ITGB6

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms a dimer with an alpha v chain and this heterodimer can bind to ligands like fibronectin and transforming growth factor beta 1. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

ITGB6: Integrin alpha-V/beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Belongs to the integrin beta chain family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: external side of plasma membrane; focal adhesion; integrin complex; plasma membrane; receptor complex

Molecular Function: integrin binding; protein binding; viral receptor activity

Biological Process: cell adhesion; cell adhesion mediated by integrin; cell-matrix adhesion; entry of virus into host cell; extracellular matrix organization; inflammatory response; integrin-mediated signaling pathway

Disease: Amelogenesis Imperfecta, Type Ih