Mouse Integrin Alpha 3 (ITGa3) ELISA Kit | ITGa3 elisa kit

Mouse Integrin Alpha 3 (ITGa3) ELISA Kit

Cat. Num. AAA2703130

• Gene Names: ITGA3; VL3A; CD49C; FRP-2; GAPB3; ILNEB; MSK18; VCA-2; VLA3a; GAP-B3
• Reactivity: Mouse
• Synonyms: Integrin Alpha 3 (ITGa3); Mouse Integrin Alpha 3 (ITGa3) ELISA Kit; CD49c; CD49-C; ITG-A3; GAP-B3; GAPB3; MSK18; VCA-2; VL3A; VLA3a; FRP-2; Galactoprotein B3; Alpha 3 Subunit Of VLA-3 Receptor; CD49 antigen-like family member C; ITGa3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 3 (ITGa3).; No significant cross-reactivity or interference between Integrin Alpha 3 (ITGa3) and analogues was observed.
• Sequence Length: 1,066

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha 3 (ITGa3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha 3 (ITGa3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGa3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 3 (ITGa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 3 (ITGa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 3 (ITGa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 3 (ITGa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504747
• NCBI GeneID: 3675
• NCBI Accession #: NP_002195.1
• NCBI GenBank Nucleotide #: NM_002204.3
• UniProt Accession #: P26006; A7E246; B7ZM80; B9EGQ1; D3DTX4; D3DTX5
• Molecular Weight: 118,756 Da
• NCBI Official Full Name: integrin alpha-3 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 3
• NCBI Official Symbol: ITGA3
• NCBI Official Synonym Symbols: VL3A; CD49C; FRP-2; GAPB3; ILNEB; MSK18; VCA-2; VLA3a; GAP-B3
• NCBI Protein Information: integrin alpha-3
• UniProt Protein Name: Integrin alpha-3
• Protein Family: Integrin
• UniProt Gene Name: ITGA3
• UniProt Synonym Gene Names: MSK18; GAPB3

NCBI Description

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function as cell surface adhesion molecules. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 3 subunit. This subunit joins with a beta 1 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. Expression of this gene may be correlated with breast cancer metastasis. [provided by RefSeq, Oct 2015]

Uniprot Description

Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.