anti-Human S100B Antibody | anti-S100B antibody

Goat anti-S100B Antibody

Cat. Num. AAA423784

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: S100B; Goat anti-S100B Antibody; S100 calcium binding protein B; NEF; S100; S100-B; S100beta; S-100 calcium-binding protein; beta chain; S-100 protein subunit beta; S100 calcium-binding protein; beta (neural); protein S100-B; anti-S100B antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 92

• Applicable Applications for anti-S100B antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:32000.; WB: Approx 10kDa band observed in Human Brain (Olfactory Bulb and Cerebellum) lysates. Recommended concentration: 1-3ug/ml.
• Immunogen Sequence: Peptide with sequence C-SHFLEEIKEQEVVD, from the internal region of the protein sequence according to NP_006263.1.
• Peptide Sequence + C: C-SHFLEEIKEQEVVD
• Epitope: Internal Region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((1ug/ml) staining of Human Olfactory Bulb (A) and Cerebellum (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 5454034
• NCBI GeneID: 6285
• NCBI Accession #: NP_006263.1
• NCBI GenBank Nucleotide #: NM_006272.2
• UniProt Accession #: P04271; D3DSN6
• Molecular Weight: 10.7kDa
• NCBI Official Full Name: protein S100-B
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B
• UniProt Protein Name: Protein S100-B
• Protein Family: Protein
• UniProt Gene Name: S100B

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.