Mouse IL-12 ELISA Kit | IL-12 elisa kit

Mouse IL-12 p70 ELISA Kit

Cat. Num. AAA9711584

• Gene Names: Il12a; p35; Ll12a; Il-12a; IL-12p35
• Reactivity: Mouse
• Synonyms: IL-12; Mouse IL-12 p70 ELISA Kit; IL12; IL 12; CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; subunit p35; interleukin 12; p35; interleukin 12A (natural killer cell stimulatory factor 1; cytotoxic lymphocytematuration factor 1; p35); interleukin-12 alpha chain; IL-12 elisa kit

• Reactivity: Mouse
• Specificity: Mouse IL-12 p70 ELISA Kit can be used to measure natural and recombinant Mouse IL-12 p70. Mouse IL-12 p70 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-12 p70. No significant cross-reactivity or interference between Mouse IL-12 p70 and analogues was observed.
• Sequence Length: 236

• Assay Type: Sandwich
• Sensitivity: <16 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?10%.
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?10%.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-12 elisa kit

Principle of the Assay: Mouse IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-12 P70 in samples. An antibody specific for IL-12 P70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 P70 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 P70 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 P70 bound in the initial step. The color development is stopped and the intensity of the color is measured.!!Background/Introduction: Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

NCBI and Uniprot Product Information

• NCBI GI #: 226874945
• NCBI GeneID: 16159
• NCBI Accession #: NP_001152896.1
• NCBI GenBank Nucleotide #: NP_001152896.1
• UniProt Accession #: P43431
• NCBI Official Full Name: interleukin-12 subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 12a
• NCBI Official Symbol: Il12a
• NCBI Official Synonym Symbols: p35; Ll12a; Il-12a; IL-12p35
• NCBI Protein Information: interleukin-12 subunit alpha
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: Il12a
• UniProt Synonym Gene Names: IL-12A; CLMF p35
• UniProt Entry Name: IL12A_MOUSE

Uniprot Description

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Cellular Component: extracellular space; interleukin-12 complex; cytoplasm; extracellular region

Molecular Function: interleukin-27 binding; growth factor activity; interleukin-12 beta subunit binding; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding

Biological Process: positive regulation of NK T cell activation; cell migration; positive regulation of cell adhesion; negative regulation of smooth muscle cell proliferation; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of T cell mediated cytotoxicity; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; defense response to protozoan; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell activation; response to UV-B; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of mononuclear cell proliferation; positive regulation of T cell differentiation; positive regulation of lymphocyte proliferation; positive regulation of T cell proliferation; immune response; cell cycle arrest