Mouse IL-12(p70) ELISA Kit | IL12A elisa kit

Mouse IL-12(p70) PicoKine ELISA Kit

Cat. Num. AAA175847

• Gene Names: Il12a; p35; Ll12a; Il-12a; IL-12p35
• Reactivity: Mouse
• Synonyms: IL-12(p70); Mouse IL-12(p70) PicoKine ELISA Kit; Interleukin-12 subunit alpha; interleukin 12A; CLMF; CLMF p35; Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 1 p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL-12A; IL12A; IL12A_HUMAN; IL35 subunit; Interleukin 12 alpha chain; Interleukin 12 p35; Interleukin 12 p70; Natural killer cell stimulatory factor 1; Natural killer cell stimulatory factor 1 35 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; P35; IL12A elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse IL-12(P70)
• Sequence Length: 236

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1.5pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse IL-12(p70) PicoKine ELISA Kit standard curve )

Related Product Information for IL12A elisa kit

Principle of the assay: mouse IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(P70) has been precoated onto 96-well plates. Standards (sf21,(M23-S335)+(R23-A215)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(P70) amount of sample captured in plate.
Background: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

NCBI and Uniprot Product Information

• NCBI GeneID: 16159
• UniProt Accession #: F8WI71; P43431
• Molecular Weight: 26,204 Da
• NCBI Official Synonym Full Names: interleukin 12a
• NCBI Official Symbol: Il12a
• NCBI Official Synonym Symbols: p35; Ll12a; Il-12a; IL-12p35
• NCBI Protein Information: interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit
• UniProt Protein Name: Interleukin-12 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il12a
• UniProt Entry Name: F8WI71_MOUSE

Uniprot Description

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Cellular Component: extracellular space; interleukin-12 complex; cytoplasm; extracellular region

Molecular Function: interleukin-27 binding; interleukin-12 beta subunit binding; growth factor activity; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding

Biological Process: cell migration; positive regulation of NK T cell activation; positive regulation of cell adhesion; positive regulation of T cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; negative regulation of smooth muscle cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; defense response to protozoan; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell activation; response to UV-B; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of T cell differentiation; positive regulation of mononuclear cell proliferation; positive regulation of lymphocyte proliferation; immune response; positive regulation of T cell proliferation; cell cycle arrest