Sheep Galectin 12 ELISA Kit | GAL12 elisa kit

Sheep Galectin 12 ELISA Kit

Cat. Num. AAA733972

• Gene Names: LGALS12; GAL12; GRIP1
• Reactivity: Sheep
• Synonyms: Galectin 12; Sheep Galectin 12 ELISA Kit; GAL12 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This GAL12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep GAL12. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAL12 elisa kit

Principle of the Assay: GAL12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAL12 antibody and an GAL12-HRP conjugate. The assay sample and buffer are incubated together with GAL12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAL12 concentration since GAL12 from samples and GAL12-HRP conjugate compete for the anti-GAL12 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAL12 from the sample, fewer sites are left to bind GAL12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAL12 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GAL12 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 216547811
• NCBI GeneID: 85329
• NCBI Accession #: NP_001136008.1
• NCBI GenBank Nucleotide #: NM_001142536.1
• Molecular Weight: 37,629 Da
• NCBI Official Full Name: galectin-12 isoform 3
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 12
• NCBI Official Symbol: LGALS12
• NCBI Official Synonym Symbols: GAL12; GRIP1
• NCBI Protein Information: galectin-12; galectin 12; galectin-related inhibitor of proliferation
• UniProt Protein Name: Galectin-12
• Protein Family: Gallinacin
• UniProt Gene Name: LGALS12
• UniProt Synonym Gene Names: GRIP1; Gal-12
• UniProt Entry Name: LEG12_HUMAN

NCBI Description

This gene encodes a member of the galectin superfamily, a group of beta-galactoside-binding proteins with conserved carbohydrate recognition domains. The related mouse protein is a primary regulator of the early stages of adipose tissue development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, May 2010]

Uniprot Description

LGALS12: Binds lactose. May participate in the apoptosis of adipocytes. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: mitochondrion; nucleus

Molecular Function: lactose binding