Mouse IgG2a ELISA Kit | IgG2a elisa kit
Mouse IgG2a (Immunoglobulin G2a) ELISA Kit
Reactivity
Mouse
Synonyms
IgG2a; Mouse IgG2a (Immunoglobulin G2a) ELISA Kit; IgG2a elisa kit
Reactivity
Mouse
Specificity
This kit recognizes natural and some recombinant Mouse IgG2a. No significant crossreactivity or interference between Mouse IgG2a and analogues was observed.
Sequence Length
108
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
7.813-500ng/mL
Sensitivity
Min: 4.688ng/mL; Max: 500ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IgG2a elisa kit
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Mouse IgG2a concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse IgG2a. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse IgG2a, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG2a. The concentration of Mouse IgG2a in samples can be calculated by comparing the OD of the samples with the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Mouse IgG2a. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Mouse IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Mouse IgG2a, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgG2a. The concentration of Mouse IgG2a in samples can be calculated by comparing the OD of the samples with the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
immunoglobulin heavy chain variable region, partial
