Mouse Histone deacetylase complex subunit SAP130 (SAP130) ELISA Kit | SAP130 elisa kit
Mouse Histone deacetylase complex subunit SAP130 (SAP130) ELISA Kit
Gene Names
Sap130; 6720406D06; 2610304F09Rik
Reactivity
Mouse
Synonyms
Histone deacetylase complex subunit SAP130 (SAP130); Mouse Histone deacetylase complex subunit SAP130 (SAP130) ELISA Kit; FLJ12761; Sin3-associated polypeptide p130|histone deacetylase complex subunit SAP130|mSin3A-associated protein 130; SAP130 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SAP130. No significant cross-reactivity or interference between Mouse SAP130 and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate SAP130 in samples. An antibody specific for SAP130 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySAP130 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAP130 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAP130 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
111,228 Da
NCBI Official Full Name
histone deacetylase complex subunit SAP130
NCBI Official Synonym Full Names
Sin3A associated protein
NCBI Official Symbol
Sap130
NCBI Official Synonym Symbols
6720406D06; 2610304F09Rik
NCBI Protein Information
histone deacetylase complex subunit SAP130; Sin3A associated protein, 130kDa; sin3-associated polypeptide p130; 130 kDa Sin3-associated polypeptide
UniProt Protein Name
Histone deacetylase complex subunit SAP130
Protein Family
UniProt Gene Name
Sap130
UniProt Entry Name
SP130_MOUSE
Uniprot Description
SAP130: Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Belongs to the SAP130 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Acetyltransferase
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; histone acetyltransferase activity; transcription coactivator activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
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Product Notes
The Mouse SAP130 sap130 (Catalog #AAA2802055) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2802055 ELISA Kit recognizes Mouse SAP130. It is sometimes possible for the material contained within the vial of "Histone deacetylase complex subunit SAP130 (SAP130), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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