Mouse Hepatic lipase (HL) ELISA Kit | HL elisa kit

Mouse Hepatic lipase (HL) ELISA Kit

Cat. Num. AAA262817

• Gene Names: LIPC; HL; HTGL; LIPH; HDLCQ12
• Reactivity: Mouse
• Synonyms: Hepatic lipase (HL); Mouse Hepatic lipase (HL) ELISA Kit; HL elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 499

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HL concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HL elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse HL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 194097335
• NCBI GeneID: 3990
• NCBI Accession #: NP_000227.2
• NCBI GenBank Nucleotide #: NM_000236.2
• Molecular Weight: 55,914 Da
• NCBI Official Full Name: hepatic triacylglycerol lipase
• NCBI Official Synonym Full Names: lipase, hepatic
• NCBI Official Symbol: LIPC
• NCBI Official Synonym Symbols: HL; HTGL; LIPH; HDLCQ12
• NCBI Protein Information: hepatic triacylglycerol lipase; hepatic lipase; lipase member C
• UniProt Protein Name: Hepatic triacylglycerol lipase
• UniProt Gene Name: LIPC
• UniProt Synonym Gene Names: HTGL; HL; Hepatic lipase
• UniProt Entry Name: LIPC_HUMAN

NCBI Description

LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Phospholipase; Secreted, signal peptide; Secreted; Lipid Metabolism - glycerolipid; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 15q21-q23

Cellular Component: extracellular space

Molecular Function: heparin binding; triacylglycerol lipase activity; low-density lipoprotein binding; apolipoprotein binding; phospholipase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol catabolic process; fatty acid biosynthetic process

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 12; Hepatic Lipase Deficiency; Diabetes Mellitus, Noninsulin-dependent