Rabbit anti-Human, Mouse ART3 Polyclonal Antibody | anti-ART3 antibody

ART3 (Ecto-ADP-ribosyltransferase 3, NAD(P)(+)-arginine ADP-ribosyltransferase 3, Mono(ADP-ribosyl) Transferase 3, TMART) (AP)

Cat. Num. AAA6370408

• Gene Names: ART3; ARTC3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ART3; Polyclonal Antibody; ART3 (Ecto-ADP-ribosyltransferase 3; NAD(P)(+)-arginine ADP-ribosyltransferase 3; Mono(ADP-ribosyl) Transferase 3; TMART) (AP); anti-ART3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ART3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ART3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ART3, aa1-378 (NP_001170.2).
• Immunogen Sequence: MKTGHFEIVTMLLATMILVDIFQVKAEVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHFYLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVDIENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVYNPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPEDKSQGNINNPTPGPVPVPGPKSHPSASSGKLLLPQFGMVIILISVSAINLFVAL
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ART3 expression in transfected 293T cell line by ART3 polyclonal antibody. Lane 1: ART3 transfected lysate (42.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ART3 antibody

Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367aa human ART3 protein has an estimated molecular mass of 41.5kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.

Product Categories/Family for anti-ART3 antibody

Antibodies; Enzymes; Ribosyltransferase

References

1. An optimized isolation of biotinylated cell surface proteins reveals novel players in cancer metastasis. Karhemo PR, Ravela S, Laakso M, Ritamo I, Tatti O, Makinen S, Goodison S, Stenman UH, Holtta E, Hautaniemi S, Valmu L, Lehti K, Laakkonen P.J Proteomics. 2012 Jul 17.

NCBI and Uniprot Product Information

• NCBI GI #: 21361167
• NCBI GeneID: 419
• NCBI Accession #: NP_001170.2
• NCBI GenBank Nucleotide #: NM_001179.2
• UniProt Accession #: Q13508; Q53XW3; Q6FHT7; Q8WVJ7; Q93069; Q96HL1
• Molecular Weight: 42,716 Da
• NCBI Official Full Name: ecto-ADP-ribosyltransferase 3 isoform b
• NCBI Official Synonym Full Names: ADP-ribosyltransferase 3
• NCBI Official Symbol: ART3
• NCBI Official Synonym Symbols: ARTC3
• NCBI Protein Information: ecto-ADP-ribosyltransferase 3; mono-ADP-ribosyltransferase; mono(ADP-ribosyl)transferase 3; NAD(P)(+)--arginine ADP-ribosyltransferase 3; ADP-ribosyltransferase C2 and C3 toxin-like 3
• UniProt Protein Name: Ecto-ADP-ribosyltransferase 3
• Protein Family: Ecto-ADP-ribosyltransferase
• UniProt Gene Name: ART3
• UniProt Synonym Gene Names: TMART; ARTC3
• UniProt Entry Name: NAR3_HUMAN

NCBI Description

This gene encodes an arginine-specific ADP-ribosyltransferase. The encoded protein catalyzes a reversible reaction which modifies proteins by the addition or removal of ADP-ribose to an arginine residue to regulate the function of the modified protein. An ADP-ribosyltransferase pseudogene is located on chromosome 11. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

ART3: an arginine-specific ADP-ribosyltransferase. The encoded protein catalyzes a reversible reaction which modifies proteins by the addition or removal of ADP-ribose to an arginine residue to regulate the function of the modified protein. An ADP-ribosyltransferase pseudogene is located on chromosome 11. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Protein type: Membrane protein, integral; Membrane protein, GPI anchor; EC 2.4.2.31; Transferase

Chromosomal Location of Human Ortholog: 4q21.1|4p15.1-p14

Cellular Component: integral to plasma membrane

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation