Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycerate mutase 1 (PGAM1) Standard Curve.)
Mouse Phosphoglycerate Mutase 1 (PGAM1) ELISA Kit | PGAM1 elisa kit
Mouse Phosphoglycerate Mutase 1 (PGAM1) ELISA Kit
Gene Names
Pgam1; Pgam-1; 2310050F24Rik
Reactivity
Mouse
Synonyms
Phosphoglycerate Mutase 1 (PGAM1); Mouse Phosphoglycerate Mutase 1 (PGAM1) ELISA Kit; PGAM1; RP11-452K12.8; PGAM-B; PGAMA; BPG-dependent PGAM 1; phosphoglycerate mutase 1; phosphoglycerate mutase A; nonmuscle form; PGAM1 elisa kit
Reactivity
Mouse
Specificity
Mouse Phosphoglycerate mutase 1 (PGAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PGAM1. No significant cross-reactivity or interference between Mouse PGAM1 and analogues was observed.
Sequence Length
254
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycerate mutase 1 (PGAM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphoglycerate mutase 1 (PGAM1) Standard Curve.)
Related Product Information for PGAM1 elisa kit
Description: This Mouse Phosphoglycerate mutase 1 (PGAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGAM1 in samples. An antibody specific for PGAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Phosphoglyceric acid mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The fact that the PGAMA and GOT1 loci are linked in the mouse supports the assignment of PGAMA to human chromosome 10. PGAM is a dimeric enzyme containing, in different tissues, different proportions of a muscle (MM) isozyme, a brain (BB) isozyme, and a hybrid form (MB). Electrophoresis of normal adult human muscle PGAM shows marked predominance of the MM band with only faint BB and MB bands. In most other human tissues, including brain, liver, erythrocytes, and leukocytes, PGAM-BB is the only demonstrable isozyme. In cardiac muscle extracts, all 3 bands are seen, although PGAM-MM predominates.
Background: Phosphoglyceric acid mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The fact that the PGAMA and GOT1 loci are linked in the mouse supports the assignment of PGAMA to human chromosome 10. PGAM is a dimeric enzyme containing, in different tissues, different proportions of a muscle (MM) isozyme, a brain (BB) isozyme, and a hybrid form (MB). Electrophoresis of normal adult human muscle PGAM shows marked predominance of the MM band with only faint BB and MB bands. In most other human tissues, including brain, liver, erythrocytes, and leukocytes, PGAM-BB is the only demonstrable isozyme. In cardiac muscle extracts, all 3 bands are seen, although PGAM-MM predominates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphoglycerate mutase 1
NCBI Official Synonym Full Names
phosphoglycerate mutase 1
NCBI Official Symbol
Pgam1
NCBI Official Synonym Symbols
Pgam-1; 2310050F24Rik
NCBI Protein Information
phosphoglycerate mutase 1
UniProt Protein Name
Phosphoglycerate mutase 1
Protein Family
UniProt Gene Name
Pgam1
UniProt Synonym Gene Names
PGAM-B
UniProt Entry Name
PGAM1_MOUSE
Uniprot Description
PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 5.4.2.11; EC 3.1.3.13; Phosphatase (non-protein); EC 5.4.2.4; Isomerase
Cellular Component: membrane; cytoplasm; cytosol; nucleus; myelin sheath
Molecular Function: intramolecular transferase activity, phosphotransferases; phosphoglycerate mutase activity; hydrolase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; isomerase activity; catalytic activity; protein kinase binding
Biological Process: respiratory burst; glycolysis; metabolic process; regulation of glycolysis; regulation of pentose-phosphate shunt
Research Articles on PGAM1
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Product Notes
The Mouse PGAM1 pgam1 (Catalog #AAA9717376) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717376 ELISA Kit recognizes Mouse PGAM1. It is sometimes possible for the material contained within the vial of "Phosphoglycerate Mutase 1 (PGAM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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