Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Group IID secretory phospholipase A2 (PLA2G2D) Standard Curve.)
Mouse Group IID Secretory Phospholipase A2 (PLA2G2D) ELISA Kit | PLA2G2D elisa kit
Mouse Group IID Secretory Phospholipase A2 (PLA2G2D) ELISA Kit
Gene Names
Pla2g2d; GIID; mGIID; Splash; PLA2IID; sPLA2-IID; GIID sPLA2
Reactivity
Mouse
Synonyms
Group IID Secretory Phospholipase A2 (PLA2G2D); Mouse Group IID Secretory Phospholipase A2 (PLA2G2D) ELISA Kit; PLA2G2D; UNQ768; SPLASH; sPLA2S; secretory phospholipase A2s; PLA2G2D elisa kit
Reactivity
Mouse
Specificity
Mouse Group IID secretory phospholipase A2 (PLA2G2D) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLA2G2D. No significant cross-reactivity or interference between Mouse PLA2G2D and analogues was observed.
Sequence Length
144
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Group IID secretory phospholipase A2 (PLA2G2D) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Group IID secretory phospholipase A2 (PLA2G2D) Standard Curve.)
Related Product Information for PLA2G2D elisa kit
Description: This Mouse Group IID secretory phospholipase A2 (PLA2G2D) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA2G2D in samples. An antibody specific for PLA2G2D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G2D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G2D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G2D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PLA2G2D, The extracellular forms of phospholipases A2 have been isolated from different venoms (snake, bee, and wasp), from virtually every studied mammalian tissue (including pancreas and kidney) as well as from bacteria. They require Ca2+ for activity.Pancreatic PLA2 serve for the initial digestion of phospholipid compounds in dietary fat. Venom phospholipases help to immobilize prey by promoting cell lysis.Analysis of enzymatic activity detected most activity in culture supernatant and determined that PLA2G2D preferentially hydrolyzes phosphatidylglycerol and phosphatidylethanolamine, followed by phosphatidylcholine, but does not hydrolyze phosphatidylserine or phosphatidic acid. Northern blot analysis revealed variable expression of 2.0- and 1.0-kb transcripts, with highest expression in pancreas and spleen.
Background: PLA2G2D, The extracellular forms of phospholipases A2 have been isolated from different venoms (snake, bee, and wasp), from virtually every studied mammalian tissue (including pancreas and kidney) as well as from bacteria. They require Ca2+ for activity.Pancreatic PLA2 serve for the initial digestion of phospholipid compounds in dietary fat. Venom phospholipases help to immobilize prey by promoting cell lysis.Analysis of enzymatic activity detected most activity in culture supernatant and determined that PLA2G2D preferentially hydrolyzes phosphatidylglycerol and phosphatidylethanolamine, followed by phosphatidylcholine, but does not hydrolyze phosphatidylserine or phosphatidic acid. Northern blot analysis revealed variable expression of 2.0- and 1.0-kb transcripts, with highest expression in pancreas and spleen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
group IID secretory phospholipase A2 isoform 1
NCBI Official Synonym Full Names
phospholipase A2, group IID
NCBI Official Symbol
Pla2g2d
NCBI Official Synonym Symbols
GIID; mGIID; Splash; PLA2IID; sPLA2-IID; GIID sPLA2
NCBI Protein Information
group IID secretory phospholipase A2
UniProt Protein Name
Group IID secretory phospholipase A2
Protein Family
UniProt Gene Name
Pla2g2d
UniProt Synonym Gene Names
Pla2a2; Splash; GIID sPLA2; sPLA2-IID
Uniprot Description
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.
Research Articles on PLA2G2D
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Product Notes
The Mouse PLA2G2D pla2g2d (Catalog #AAA9717349) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717349 ELISA Kit recognizes Mouse PLA2G2D. It is sometimes possible for the material contained within the vial of "Group IID Secretory Phospholipase A2 (PLA2G2D), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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