Mouse Granzymes A (GZMA) ELISA Kit | GZMA elisa kit

Mouse Granzymes A (GZMA) ELISA Kit

Cat. Num. AAA287127

• Gene Names: Gzma; Hf; Hf1; SE1; TSP1; Ctla3; TSP-1; Ctla-3; AW494114
• Reactivity: Mouse
• Synonyms: Granzymes A (GZMA); Mouse Granzymes A (GZMA) ELISA Kit; CTLA3; HFSP; CTL tryptase; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); granzyme A; GZMA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse GZMA. No significant cross-reactivity or interference between Mouse GZMA and analogues was observed.
• Sequence Length: 260

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GZMA elisa kit

This assay employs a two-site sandwich ELISA to quantitate GZMA in samples. An antibody specific for GZMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GZMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GZMA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GZMA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6754102
• NCBI GeneID: 14938
• NCBI Accession #: NP_034500.1
• NCBI GenBank Nucleotide #: NM_010370.2
• UniProt Accession #: P11032; P15118
• Molecular Weight: 28,541 Da
• NCBI Official Full Name: granzyme A
• NCBI Official Synonym Full Names: granzyme A
• NCBI Official Symbol: Gzma
• NCBI Official Synonym Symbols: Hf; Hf1; SE1; TSP1; Ctla3; TSP-1; Ctla-3; AW494114
• NCBI Protein Information: granzyme A
• UniProt Protein Name: Granzyme A
• Protein Family: Granzyme
• UniProt Gene Name: Gzma
• UniProt Synonym Gene Names: Ctla-3; Ctla3; Mtsp-1; TSP-1

Uniprot Description

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA ().