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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SH2D2A protein (red) or empty vector (blue) stained using SH2D2A antibody)

Mouse SH2D2A Monoclonal Antibody | anti-SH2D2A antibody

SH2D2A antibody

Gene Names
SH2D2A; SCAP; TSAD; VRAP; F2771
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
SH2D2A antibody was purified by affinity chromatography.
Synonyms
SH2D2A; Monoclonal Antibody; SH2D2A antibody; Monoclonal SH2D2A; Anti-SH2D2A; F2771; SCAP; TSAD; VRAP; SHDA 2; SHDA-2; anti-SH2D2A antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C7
Purity/Purification
SH2D2A antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
389
Applicable Applications for anti-SH2D2A antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:500-2000
Biological Significance
This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants.
Cross-Reactivity
Human, Monkey, Mouse
Immunogen
SH2D2A antibody was raised in Mouse using full length human recombinant protein of human SH2D2A produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant SH2D2A protein (red) or empty vector (blue) stained using SH2D2A antibody)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant SH2D2A protein (red) or empty vector (blue) stained using SH2D2A antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SH2D2A protein (Right) or empty vector (Left) detected with SH2D2A antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SH2D2A protein (Right) or empty vector (Left) detected with SH2D2A antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SH2D2A protein using SH2D2A antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SH2D2A protein using SH2D2A antibody)
Related Product Information for anti-SH2D2A antibody
Mouse monoclonal SH2D2A antibody
Product Categories/Family for anti-SH2D2A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,954 Da
NCBI Official Full Name
SH2 domain-containing protein 2A isoform 2
NCBI Official Synonym Full Names
SH2 domain containing 2A
NCBI Official Symbol
SH2D2A
NCBI Official Synonym Symbols
SCAP; TSAD; VRAP; F2771
NCBI Protein Information
SH2 domain-containing protein 2A
UniProt Protein Name
SH2 domain-containing protein 2A
UniProt Gene Name
SH2D2A
UniProt Synonym Gene Names
SCAP; TSAD; VRAP; TSAd
UniProt Entry Name
SH22A_HUMAN

NCBI Description

This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

TSAd: an adaptor protein that may facilitate and regulate interaction of KDR with effector proteins important to endothelial cell survival and proliferation. May play a role in the CD4-p56- LCK-dependent signal transduction pathway in T cells. Could also play an important role in normal and pathological angiogenesis.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; SH3 domain binding

Biological Process: cell proliferation; positive regulation of signal transduction; angiogenesis; vascular endothelial growth factor receptor signaling pathway; cell differentiation; signal transduction

Research Articles on SH2D2A

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Product Notes

The SH2D2A sh2d2a (Catalog #AAA833517) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SH2D2A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:500-2000. Researchers should empirically determine the suitability of the SH2D2A sh2d2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH2D2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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