Mouse Glutathione S Transferase Omega 1 (GSTo1) ELISA Kit | GSTo1 elisa kit

Mouse Glutathione S Transferase Omega 1 (GSTo1) ELISA Kit

Cat. Num. AAA2022990

• Gene Names: GSTO1; P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21
• Reactivity: Mouse
• Synonyms: Glutathione S Transferase Omega 1 (GSTo1); Mouse Glutathione S Transferase Omega 1 (GSTo1) ELISA Kit; P28; GSTTLp28; GSTw1; SPG-R; Glutathione-dependent dehydroascorbate reductase; Monomethylarsonic acid reductase; MMA(V) reductase; S-(Phenacyl)glutathione reductase; GSTo1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Omega 1 (GSTo1).; No significant cross-reactivity or interference between Glutathione S Transferase Omega 1 (GSTo1) and analogues was observed.
• Sequence Length: 213

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Omega 1 (GSTo1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Omega 1 (GSTo1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSTo1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Omega 1 (GSTo1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Omega 1 (GSTo1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Omega 1 (GSTo1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Omega 1 (GSTo1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GSTo1 elisa kit

Enzyme & Kinase; Metabolic pathway; Tumor immunity; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 12652753
• NCBI GeneID: 9446
• UniProt Accession #: P78417; Q5TA03; Q7Z3T2; D3DRA3; F5H7H0
• Molecular Weight: 24,796 Da
• NCBI Official Full Name: Glutathione S-transferase omega 1
• NCBI Official Synonym Full Names: glutathione S-transferase omega 1
• NCBI Official Symbol: GSTO1
• NCBI Official Synonym Symbols: P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21
• NCBI Protein Information: glutathione S-transferase omega-1
• UniProt Protein Name: Glutathione S-transferase omega-1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTO1
• UniProt Synonym Gene Names: GSTTLP28; GSTO-1; GSTO 1-1; MMA(V) reductase; SPG-R
• UniProt Entry Name: GSTO1_HUMAN

NCBI Description

The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

GSTO1: Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. Belongs to the GST superfamily. Omega family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.20.4.2; Transferase; EC 1.8.5.1; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18

Chromosomal Location of Human Ortholog: 10q25.1

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; glutathione transferase activity; glutathione dehydrogenase (ascorbate) activity; methylarsonate reductase activity; oxidoreductase activity

Biological Process: positive regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic metabolic process; L-ascorbic acid metabolic process; xenobiotic catabolic process