Mouse GAS1 ELISA Kit | GAS1 elisa kit

Mouse GAS1 PicoKine ELISA Kit

Cat. Num. AAA1752650

• Gene Names: Gas1; Gas-1; AW554192
• Reactivity: Mouse
• Synonyms: GAS1; Mouse GAS1 PicoKine ELISA Kit; Mouse GAS1; growth arrest specific 1; Growth arrest-specific protein 1; GAS-1; Gas1; Gas-1; GAS1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural rat CASP-3. No significant cross-reactivity or interference was observed.
• Sequence Length: 384

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 0.31 ng/mL -20.0 ng/mL
• Sensitivity: <0.078 ng/mL
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAS1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat caspase-3,CASP-3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CASP-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CASP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CASP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6679941
• NCBI GeneID: 14451
• NCBI Accession #: NP_032112.1
• NCBI GenBank Nucleotide #: NP_032112.1
• UniProt Accession #: Q01721
• NCBI Official Full Name: growth arrest-specific protein 1
• NCBI Official Synonym Full Names: growth arrest specific 1
• NCBI Official Symbol: Gas1
• NCBI Official Synonym Symbols: Gas-1; AW554192
• NCBI Protein Information: growth arrest-specific protein 1
• UniProt Protein Name: Growth arrest-specific protein 1
• Protein Family: Germacrene A synthase
• UniProt Gene Name: Gas1
• UniProt Synonym Gene Names: Gas-1; GAS-1
• UniProt Entry Name: GAS1_MOUSE

Uniprot Description

GAS1: Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.

Protein type: Membrane protein, GPI anchor

Cellular Component: anchored to plasma membrane; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: developmental growth; axon guidance; embryonic skeletal development; programmed cell death; cerebellum morphogenesis; middle ear morphogenesis; palate development; odontogenesis; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; negative regulation of mitotic cell cycle; embryonic morphogenesis; cell cycle arrest; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; eye morphogenesis; cell fate commitment; camera-type eye development; outer ear morphogenesis; embryonic cranial skeleton morphogenesis; cell cycle; dorsal/ventral pattern formation; negative regulation of smoothened signaling pathway; embryonic digit morphogenesis; negative regulation of cell growth; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; regulation of smoothened signaling pathway