Mouse Proline-rich AKT1 substrate 1 ELISA Kit | Akt1s1 elisa kit

Mouse Proline-rich AKT1 substrate 1 ELISA Kit

Cat. Num. AAA2880481

• Gene Names: Akt1s1; Lobe; Lobel; PRAS40; AI227026; 1110012J22Rik
• Reactivity: Mouse
• Synonyms: Proline-rich AKT1 substrate 1; Mouse Proline-rich AKT1 substrate 1 ELISA Kit; Proline-rich AKT substrate; Akt1s1; Pras; Akt1s1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Proline-rich AKT substrate.. No significant cross-reactivity or interference was observed.
• Sequence Length: 257

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 0.31 ng/mL - 20.0 ng/mL
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Akt1s1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Proline-rich AKT substrate concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proline-rich AKT substrate. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Proline-rich AKT substrate and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proline-rich AKT substrate, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Proline-rich AKT substrate in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 359807125
• NCBI GeneID: 67605
• NCBI Accession #: NP_001240849.1
• NCBI GenBank Nucleotide #: NM_001253920.1
• UniProt Accession #: Q9D1F4; A2RT52
• Molecular Weight: 27,483 Da
• NCBI Official Full Name: proline-rich AKT1 substrate 1 isoform b
• NCBI Official Synonym Full Names: AKT1 substrate 1 (proline-rich)
• NCBI Official Symbol: Akt1s1
• NCBI Official Synonym Symbols: Lobe; Lobel; PRAS40; AI227026; 1110012J22Rik
• NCBI Protein Information: proline-rich AKT1 substrate 1
• UniProt Protein Name: Proline-rich AKT1 substrate 1
• Protein Family: Proline-rich AKT1 substrate
• UniProt Gene Name: Akt1s1
• UniProt Synonym Gene Names: Proline-rich AKT substrate
• UniProt Entry Name: AKTS1_MOUSE

Uniprot Description

PRAS40: plays a role in phosphatidylinositol 3-kinase (PI3K)/AKT survival signaling. PRAS40 is a physiological target of in vivo insulin action. Hyperinsulinemia increased its phosphorylation in human skeletal muscle biopsies. Phosphorylated PRAS40 is predominantly localized to the nucleus. In rats fed a high-fat diet (HFD), phosphorylation of PRAS40 was markedly reduced when compared with low-fat diet-fed animals in all tissues examined. A novel mTOR binding partner that mediates Akt signals to mTOR. Binding of PRAS40 inhibits mTOR activity and suppresses constitutive activation of mTOR in cells lacking TSC2. Phosphorylation by Akt and perhaps related kinases leads to its binding to 14-3-3. Two alternatively spliced isoforms have been described.

Protein type: Apoptosis

Cellular Component: cytoplasm; cytosol; nucleus; protein complex

Biological Process: negative regulation of cell size; negative regulation of protein kinase activity; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; Notch signaling pathway; regulation of neuron apoptosis