Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit | FYN elisa kit

Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit

Cat. Num. AAA453352

• Gene Names: Fyn; AI448320; AW552119
• Reactivity: Mouse
• Synonyms: FYN Oncogene Related To SRC/FGR/YES (FYN); Mouse FYN Oncogene Related To SRC/FGR/YES (FYN) ELISA Kit; SLK; SYN; c-Fyn; Src-like kinase; FYN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FYN. No significant cross-reactivity or interference between FYN and analogues was observed.
• Sequence Length: 534

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FYN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FYN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FYN elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FYN in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FYN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FYN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FYN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FYN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 171543839
• NCBI GeneID: 14360
• NCBI Accession #: NP_001116364.1
• NCBI GenBank Nucleotide #: NM_001122892.1
• UniProt Accession #: P39688; Q3TAT3; Q3U0T5; Q8K2A3
• Molecular Weight: 60,059 Da
• NCBI Official Full Name: tyrosine-protein kinase Fyn isoform b
• NCBI Official Synonym Full Names: Fyn proto-oncogene
• NCBI Official Symbol: Fyn
• NCBI Official Synonym Symbols: AI448320; AW552119
• NCBI Protein Information: tyrosine-protein kinase Fyn
• UniProt Protein Name: Tyrosine-protein kinase Fyn
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Fyn

Uniprot Description

Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1 ().