Human C-Mer Proto Oncogene Tyrosine Kinase (MERTK) ELISA Kit | MERTK elisa kit

Human C-Mer Proto Oncogene Tyrosine Kinase (MERTK) ELISA Kit

Cat. Num. AAA2705436

• Gene Names: Mertk; Eyk; Mer; Nyk; nmf12
• Reactivity: Human
• Synonyms: C-Mer Proto Oncogene Tyrosine Kinase (MERTK); Human C-Mer Proto Oncogene Tyrosine Kinase (MERTK) ELISA Kit; MER; c-Mer; RP38; MERTK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of C-Mer Proto Oncogene Tyrosine Kinase (MERTK).; No significant cross-reactivity or interference between C-Mer Proto Oncogene Tyrosine Kinase (MERTK) and analogues was observed.
• Sequence Length: 994

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Mer Proto Oncogene Tyrosine Kinase (MERTK) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Mer Proto Oncogene Tyrosine Kinase (MERTK) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MERTK elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Mer Proto Oncogene Tyrosine Kinase (MERTK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Mer Proto Oncogene Tyrosine Kinase (MERTK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Mer Proto Oncogene Tyrosine Kinase (MERTK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Mer Proto Oncogene Tyrosine Kinase (MERTK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148696270
• NCBI GeneID: 17289
• UniProt Accession #: Q60805; Q62194
• Molecular Weight: 110,157 Da
• NCBI Official Full Name: c-mer proto-oncogene tyrosine kinase
• NCBI Official Synonym Full Names: c-mer proto-oncogene tyrosine kinase
• NCBI Official Symbol: Mertk
• NCBI Official Synonym Symbols: Eyk; Mer; Nyk; nmf12
• NCBI Protein Information: tyrosine-protein kinase Mer
• UniProt Protein Name: Tyrosine-protein kinase Mer
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Mertk
• UniProt Synonym Gene Names: Mer

Uniprot Description

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.