Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Forkhead box protein C2 (FOXC2) Standard Curve.)
Mouse Forkhead Box Protein C2 (FOXC2) ELISA Kit | FOXC2 elisa kit
Mouse Forkhead Box Protein C2 (FOXC2) ELISA Kit
Gene Names
Foxc2; Mfh1; Fkh14; MFH-1; Hfhbf3
Reactivity
Mouse
Synonyms
Forkhead Box Protein C2 (FOXC2); Mouse Forkhead Box Protein C2 (FOXC2) ELISA Kit; FOXC2; FKHL14; LD; MFH-1; MFH1; mesenchyme forkhead 1; forkhead box C2; forkhead; Drosophila; homolog-like 14; FOXC2 elisa kit
Reactivity
Mouse
Specificity
Mouse Forkhead box protein C2 (FOXC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse FOXC2. No significant cross-reactivity or interference between Mouse FOXC2 and analogues was observed.
Sequence Length
494
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Forkhead box protein C2 (FOXC2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Forkhead box protein C2 (FOXC2) Standard Curve.)
Related Product Information for FOXC2 elisa kit
Description: This Mouse Forkhead box protein C2 (FOXC2) ELISA Kit employs a two-site sandwich ELISA to quantitate FOXC2 in samples. An antibody specific for FOXC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FOXC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FOXC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FOXC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: FOXC2 is also involved in cancer metastases. Particularly, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. EMT can be induced by a number of genes including Snail, Twist, Goosecoid, and TGF-beta 1. Suppression of FOXC2 expression using shRNA in a highly metastatic breast cancer model blocks their metastatic ability. The nature of these Foxc2-generated responses was consistent with protection against obesity and related symptoms, such as diet-induced insulin resistance. Furthermore, in wildtype mice, Foxc2 mRNA levels were upregulated by high-fat diet, whereas mice with targeted disruption of 1 Foxc2 allele had a decreased interscapular BAT cell mass.
Background: FOXC2 is also involved in cancer metastases. Particularly, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. EMT can be induced by a number of genes including Snail, Twist, Goosecoid, and TGF-beta 1. Suppression of FOXC2 expression using shRNA in a highly metastatic breast cancer model blocks their metastatic ability. The nature of these Foxc2-generated responses was consistent with protection against obesity and related symptoms, such as diet-induced insulin resistance. Furthermore, in wildtype mice, Foxc2 mRNA levels were upregulated by high-fat diet, whereas mice with targeted disruption of 1 Foxc2 allele had a decreased interscapular BAT cell mass.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
forkhead box protein C2
NCBI Official Synonym Full Names
forkhead box C2
NCBI Official Symbol
Foxc2
NCBI Official Synonym Symbols
Mfh1; Fkh14; MFH-1; Hfhbf3
NCBI Protein Information
forkhead box protein C2
UniProt Protein Name
Forkhead box protein C2
Protein Family
UniProt Gene Name
Foxc2
UniProt Synonym Gene Names
Fkh14; Fkhl14; Mfh1; BF-3; MFH-1 protein
UniProt Entry Name
FOXC2_MOUSE
Research Articles on FOXC2
Similar Products
Product Notes
The Mouse FOXC2 foxc2 (Catalog #AAA9718095) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718095 ELISA Kit recognizes Mouse FOXC2. It is sometimes possible for the material contained within the vial of "Forkhead Box Protein C2 (FOXC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
