Western Blot (WB)
(Western blot analysis of NARG1 expression in 293T whole cell lysates (lane 1). NARG1 at 101KD was detected using rabbit anti- NARG1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit anti-Human, Mouse NARG1 Polyclonal Antibody | anti-NAA15 antibody
Anti-NARG1 Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of NARG1 expression in 293T whole cell lysates (lane 1). NARG1 at 101KD was detected using rabbit anti- NARG1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of NARG1 expression in 293T whole cell lysates (lane 1). NARG1 at 101KD was detected using rabbit anti- NARG1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Immunohistochemistry (IHC)
(NARG1 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- NARG1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Immunohistochemistry (IHC)
(NARG1 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- NARG1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )
Background: NMDA receptor-regulated protein 1 (NARG1), also known as GA19 or Tbdn100 is a protein that in humans is encoded by the NAA15 gene. It is mapped to chromosome 4. NARG1 is the auxiliary subunit of the NatA (Nalpha-acetyltransferase A) complex. Both, Naa15 and Naa16 interact with the ribosome in yeast (via the ribosomal proteins, uL23 and uL29), humans and rat, thereby linking the NatA/Naa10 to the ribosome and facilitating co-translational acetylation of nascent polypeptide chains as they emerges from the exit tunnel. Furthermore, Naa15 might act as a scaffold for other factors, including the chaperone like protein HYPK (Huntingtin Interacting Protein K) and Naa50, the catalytic acetyltransferase subunit of NatE.
2. Fluge, O., Bruland, O., Akslen, L. A., Varhaug, J. E., Lillehaug, J. R. NATH, a novel gene overexpressed in papillary thyroid carcinomas. Oncogene 21: 5056-5068, 2002.
3. Sugiura, N., Patel, R. G., Corriveau, R. A. N-methyl-D-aspartate receptors regulate a group of transiently expressed genes in the developing brain. J. Biol. Chem. 276: 14257-14263, 2001.
NCBI and Uniprot Product Information
NCBI Description
N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes the auxillary subunit of the N-terminal acetyltransferase A (NatA) complex. [provided by RefSeq, Jan 2017]
Uniprot Description
NAA15: The NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Acetyltransferase
Chromosomal Location of Human Ortholog: 4q31.1
Cellular Component: cytoplasm; membrane; nucleus; transcription factor complex
Molecular Function: acetyltransferase activity; peptide alpha-N-acetyltransferase activity; protein binding; ribosome binding
Biological Process: N-terminal peptidyl-methionine acetylation; N-terminal protein amino acid acetylation; negative regulation of apoptosis; positive regulation of transcription, DNA-dependent; protein stabilization
Research Articles on NAA15
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Product Notes
The NAA15 naa15 (Catalog #AAA178695) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NARG1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NARG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: 0.1-0.5ug/ml Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml. Researchers should empirically determine the suitability of the NAA15 naa15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NARG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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