Mouse Factor XII total ELISA Kit | F12 elisa kit

Mouse Factor XII total antigen assay ELISA kit

Cat. Num. AAA135943

• Gene Names: F12; HAF; FXII
• Reactivity: Mouse
• Synonyms: Factor XII total; Mouse Factor XII total antigen assay ELISA kit; F12 elisa kit

• Reactivity: Mouse
• Form/Format: Complete kit
• Sequence Length: 597

• Source Info: Multiple
• Target Info: Factor XII
• Preparation and Storage: Recommended Storage: 4 degree C; Minimum Shelf Life: 12 months from manufacture, see label for expiration date; Shipping Condition: Cold pack

Related Product Information for F12 elisa kit

Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a severe defect in thrombus formation. The sensitive quantitative measurement of total mouse Factor XII antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Factor XII and XIIa will be detected by the assay. The concentration of Factor XII in normal human plasma ranges from 15-47 ug/ml. Normal values of Factor XII in mouse plasma have not been conclusively determined but are believed to be similar to human plasma based on in-house testing. The assay measures total mouse Factor XII in the 0.1-100 ng/ml range. Samples giving mouse Factor XII levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:50,000 to 1:400,000 dilution for plasma is suggested for best results. Mouse Factor XII will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of Factor XII in the samples. A standard calibration curve is prepared using dilutions of purified Factor XII and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Product Categories/Family for F12 elisa kit

CLOTTING FACTOR ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 171906584
• NCBI GeneID: 58992
• NCBI Accession #: NP_067464.2
• NCBI GenBank Nucleotide #: NM_021489.2
• UniProt Accession #: Q80YC5; O35727; Q6PER0
• Molecular Weight: 65,701 Da
• NCBI Official Full Name: coagulation factor XII
• NCBI Official Synonym Full Names: coagulation factor XII (Hageman factor)
• NCBI Official Symbol: F12
• NCBI Official Synonym Symbols: HAF; FXII
• NCBI Protein Information: coagulation factor XII; hageman factor
• UniProt Protein Name: Coagulation factor XII
• Protein Family: Coagulation factor
• UniProt Gene Name: F12
• UniProt Synonym Gene Names: HAF
• UniProt Entry Name: FA12_MOUSE

NCBI Description

This gene encodes a glycoprotein coagulation factor that plays an important role in the intrinsic pathway of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that is autoactivated upon contact with negatively charged surfaces or misfolded protein aggregates. Mice lacking the encoded protein have a severe defect in forming stable fibrin clots. [provided by RefSeq, Apr 2015]

Uniprot Description

F12: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta- factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Defects in F12 are the cause of factor XII deficiency (FA12D); also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3); also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted, signal peptide; EC 3.4.21.38; Secreted; Chaperone

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: protein autoprocessing; fibrinolysis; response to misfolded protein; positive regulation of blood coagulation; hemostasis; plasma kallikrein-kinin cascade; regulation of blood coagulation; positive regulation of fibrinolysis; protein processing; zymogen activation; proteolysis; blood coagulation; Factor XII activation