Typical Testing Data/Standard Curve (for reference only)
General Factor XII total antigen assay ELISA Kit | F12 elisa kit
Human Factor XII total antigen assay ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Human Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, anti-human Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor XII present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor XII. Color development is proportional to the concentration of Factor XII in the samples.
Background: Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen [1]. Factor XII is activated by kallikrein [2]. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a 'severe defect' in thrombus formation [3].
NCBI and Uniprot Product Information
NCBI Description
This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]
Uniprot Description
F12: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta- factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Defects in F12 are the cause of factor XII deficiency (FA12D); also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3); also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. Belongs to the peptidase S1 family.
Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.38; Chaperone; Protease
Chromosomal Location of Human Ortholog: 5q35.3
Cellular Component: extracellular space; plasma membrane; extracellular region
Molecular Function: protein binding; serine-type endopeptidase activity; misfolded protein binding
Biological Process: protein autoprocessing; response to misfolded protein; fibrinolysis; positive regulation of blood coagulation; plasma kallikrein-kinin cascade; innate immune response; positive regulation of fibrinolysis; zymogen activation; protein processing; proteolysis; blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation
Disease: Angioedema, Hereditary, Type Iii; Factor Xii Deficiency
Research Articles on F12
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Product Notes
The General F12 f12 (Catalog #AAA135535) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135535 ELISA Kit recognizes General F12. It is sometimes possible for the material contained within the vial of "Factor XII total antigen assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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