Mouse Ephrin B2 (EFNB2) ELISA Kit | EFNB2 elisa kit

Mouse Ephrin B2 (EFNB2) ELISA Kit

Cat. Num. AAA451558

• Gene Names: Efnb2; Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1
• Reactivity: Mouse
• Synonyms: Ephrin B2 (EFNB2); Mouse Ephrin B2 (EFNB2) ELISA Kit; EPLG5; HTKL; Htk-L; LERK5; HTK Ligand; Ligand Of Eph-Related Kinase 5; Eph-Related Receptor Tyrosine Kinase Ligand 5; EFNB2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of EFNB2. No significant cross-reactivity or interference between EFNB2 and analogues was observed.
• Sequence Length: 336

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EFNB2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EFNB2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EFNB2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EFNB2 in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EFNB2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EFNB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EFNB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EFNB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31542597
• NCBI GeneID: 13642
• NCBI Accession #: NP_034241.2
• NCBI GenBank Nucleotide #: NM_010111.5
• UniProt Accession #: P52800
• Molecular Weight: 37,202 Da
• NCBI Official Full Name: ephrin-B2
• NCBI Official Synonym Full Names: ephrin B2
• NCBI Official Symbol: Efnb2
• NCBI Official Synonym Symbols: Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1
• NCBI Protein Information: ephrin-B2
• UniProt Protein Name: Ephrin-B2
• Protein Family: Ephrin
• UniProt Gene Name: Efnb2
• UniProt Synonym Gene Names: Elf2; Epl5; Eplg5; Htkl; Lerk5; LERK-5; HTK-L

Uniprot Description

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.