Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Eosinophil cationic protein (ECP) Standard Curve.)
Mouse Eosinophil Cationic Protein (ECP) ELISA Kit | ECP elisa kit
Mouse Eosinophil Cationic Protein (ECP) ELISA Kit
Gene Names
RNASE3; ECP; RAF1; RNS3
Reactivity
Mouse
Synonyms
Eosinophil Cationic Protein (ECP); Mouse Eosinophil Cationic Protein (ECP) ELISA Kit; ECP elisa kit
Reactivity
Mouse
Specificity
Mouse Eosinophil cationic protein (ECP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ECP. No significant cross-reactivity or interference between Mouse ECP and analogues was observed.
Sequence Length
160
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Eosinophil cationic protein (ECP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Eosinophil cationic protein (ECP) Standard Curve.)
Related Product Information for ECP elisa kit
Description: This Mouse Eosinophil cationic protein (ECP) ELISA Kit employs a two-site sandwich ELISA to quantitate ECP in samples. An antibody specific for ECP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ECP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Three immunization procedures were compared for the production of antibodies to the minor components of a complex E. coli protein (ECP) mixture: a conventional protocol and two methods that allow for the selective in vitro (cascade) or in vivo (passive) depletion of highly immunogenic proteins.An E. coli protein, designated omega, has been purified at least 1000-fold. Treatment of a eovalently closed DNA duplex containing negative superhelical turns with omega results in the loss of most of the superhelical turns. The loss of superhelical turns follows a gradual course rather than a one-hit mechanism. This reaction does not require a cofactor. No other change in the physical properties of the DNA could be detected. omega is capable of introducing a "swivel" reversibly into a DNA. A plausible mechanism is postulated.
Background: Three immunization procedures were compared for the production of antibodies to the minor components of a complex E. coli protein (ECP) mixture: a conventional protocol and two methods that allow for the selective in vitro (cascade) or in vivo (passive) depletion of highly immunogenic proteins.An E. coli protein, designated omega, has been purified at least 1000-fold. Treatment of a eovalently closed DNA duplex containing negative superhelical turns with omega results in the loss of most of the superhelical turns. The loss of superhelical turns follows a gradual course rather than a one-hit mechanism. This reaction does not require a cofactor. No other change in the physical properties of the DNA could be detected. omega is capable of introducing a "swivel" reversibly into a DNA. A plausible mechanism is postulated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
eosinophil cationic protein
NCBI Official Synonym Full Names
ribonuclease A family member 3
NCBI Official Symbol
RNASE3
NCBI Official Synonym Symbols
ECP; RAF1; RNS3
NCBI Protein Information
eosinophil cationic protein
Protein Family
NCBI Description
The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
Research Articles on ECP
Similar Products
Product Notes
The Mouse ECP (Catalog #AAA9718167) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718167 ELISA Kit recognizes Mouse ECP. It is sometimes possible for the material contained within the vial of "Eosinophil Cationic Protein (ECP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
