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Western Blot (WB) (Western blot analysis of extracts of K-562 cells, using Hemoglobin epsilon antibody.)

Rabbit Hemoglobin epsilon Polyclonal Antibody | anti-HBE1 antibody

Hemoglobin epsilon Antibody

Gene Names
HBE1; HBE
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Hemoglobin epsilon; Polyclonal Antibody; Hemoglobin epsilon Antibody; Epsilon-globin; HBE_HUMAN; HBE1; Hemoglobin epsilon chain; Hemoglobin subunit epsilon; anti-HBE1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Hemoglobin epsilon antibody detects endogenous levels of Hemoglobin epsilon.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
147
Applicable Applications for anti-HBE1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human Hemoglobin epsilon.
Tissue Specificity
Red blood cells.
Predicted Cross Reactivity
Pig, Bovine, Rabbit
Similarity
Pig (91%), Bovine (82%), Rabbit (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of K-562 cells, using Hemoglobin epsilon antibody.)

Western Blot (WB) (Western blot analysis of extracts of K-562 cells, using Hemoglobin epsilon antibody.)

Immunohistochemistry (IHC)

(MBS9603505 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9603505 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-HBE1 antibody
Function: The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.
Subunit Structure: Heterotetramer of two alpha chains and two epsilon chains in early embryonic hemoglobin Gower-2; two zeta chains and two epsilon chains in early embryonic hemoglobin Gower-1.
Similarity: Belongs to the globin family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 16 kDa
Predicted: 17 kDa
NCBI Official Full Name
hemoglobin subunit epsilon
NCBI Official Synonym Full Names
hemoglobin subunit epsilon 1
NCBI Official Symbol
HBE1
NCBI Official Synonym Symbols
HBE
NCBI Protein Information
hemoglobin subunit epsilon
UniProt Protein Name
Hemoglobin subunit epsilon
Protein Family
UniProt Gene Name
HBE1
UniProt Synonym Gene Names
HBE

NCBI Description

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008]

Uniprot Description

The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.

Research Articles on HBE1

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Product Notes

The HBE1 hbe1 (Catalog #AAA9603505) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hemoglobin epsilon Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hemoglobin epsilon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HBE1 hbe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemoglobin epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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