Chicken eosinophil cationic protein (ECP) ELISA Kit | ECP elisa kit

Chicken eosinophil cationic protein (ECP)

Cat. Num. AAA2600407

• Gene Names: RNASE3; ECP; RNS3
• Reactivity: Chicken
• Synonyms: eosinophil cationic protein (ECP); Chicken eosinophil cationic protein (ECP); ECP elisa kit

• Reactivity: Chicken
• Specificity: No cross-reaction with other factors.
• Sequence Length: 160

• Samples: Chicken serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ECP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken ECP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 45243507
• NCBI GeneID: 6037
• NCBI Accession #: NP_002926.2
• NCBI GenBank Nucleotide #: NM_002935.2
• Molecular Weight: 18,385 Da
• NCBI Official Full Name: eosinophil cationic protein
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 3
• NCBI Official Symbol: RNASE3
• NCBI Official Synonym Symbols: ECP; RNS3
• NCBI Protein Information: eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease
• UniProt Protein Name: Eosinophil cationic protein
• Protein Family: Eosinophil cationic protein
• UniProt Gene Name: RNASE3
• UniProt Synonym Gene Names: ECP; RNS3; ECP; RNase 3
• UniProt Entry Name: ECP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; Ribonuclease; Secreted; EC 3.1.27.-

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: nucleic acid binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response