Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) Standard Curve.)
Mouse Endocrine Gland Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit | EG-VEGF elisa kit
Mouse Endocrine Gland Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit
Gene Names
Prok1; EG-VEGF
Reactivity
Mouse
Synonyms
Endocrine Gland Vascular Endothelial Growth Factor (EG-VEGF); Mouse Endocrine Gland Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit; PROK1; EGVEGF; PK1; PRK1; black mamba toxin-related protein; mambakine; EG-VEGF elisa kit
Reactivity
Mouse
Specificity
Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PROK1. No significant cross-reactivity or interference between Mouse PROK1 and analogues was observed.
Sequence Length
105
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) Standard Curve.)
Related Product Information for EG-VEGF elisa kit
Description: This Mouse Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PROK1 in samples. An antibody specific for PROK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The prokineticin 1 encoded by Prok1 induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.
Background: The prokineticin 1 encoded by Prok1 induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prokineticin-1 isoform 1
NCBI Official Synonym Full Names
prokineticin 1
NCBI Official Symbol
Prok1
NCBI Official Synonym Symbols
EG-VEGF
NCBI Protein Information
prokineticin-1
UniProt Protein Name
Prokineticin-1
UniProt Gene Name
Prok1
UniProt Entry Name
PROK1_MOUSE
Uniprot Description
PROK1: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Belongs to the AVIT (prokineticin) family.
Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide
Cellular Component: extracellular region
Molecular Function: G-protein-coupled receptor binding; growth factor activity
Biological Process: circadian rhythm; activation of MAPK activity; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; regulation of angiogenesis
Research Articles on EG-VEGF
Similar Products
Product Notes
The Mouse EG-VEGF prok1 (Catalog #AAA9717261) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717261 ELISA Kit recognizes Mouse EG-VEGF. It is sometimes possible for the material contained within the vial of "Endocrine Gland Vascular Endothelial Growth Factor (EG-VEGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
