Western Blot (WB)
(Figure 1. Western blot analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human T47D whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: rat brain tissue lysatesLane 4: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DGKZ/DGK-zeta antigen affinity purified polyclonal antibody (Catalog # MBS1753738) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DGKZ/DGK-zeta at approximately 124KD. The expected band size for DGKZ/DGK-zeta is at 124KD. )
Rabbit DGKZ/DGK-zeta Polyclonal Antibody | anti-DGKZ antibody
Anti-DGKZ/DGK-zeta Antibody
IHC-P: 2-5ug/ml|Human, Mouse|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human T47D whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: rat brain tissue lysatesLane 4: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DGKZ/DGK-zeta antigen affinity purified polyclonal antibody (Catalog # MBS1753738) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DGKZ/DGK-zeta at approximately 124KD. The expected band size for DGKZ/DGK-zeta is at 124KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human T47D whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: rat brain tissue lysatesLane 4: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DGKZ/DGK-zeta antigen affinity purified polyclonal antibody (Catalog # MBS1753738) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DGKZ/DGK-zeta at approximately 124KD. The expected band size for DGKZ/DGK-zeta is at 124KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).DGKZ/DGK-zeta was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).DGKZ/DGK-zeta was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).DGKZ/DGK-zeta was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).DGKZ/DGK-zeta was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).DGKZ/DGK-zeta was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of DGKZ/DGK-zeta using anti-DGKZ/DGK-zeta antibody (MBS1753738).DGKZ/DGK-zeta was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of HL-60 cells using anti-DGKZ/DGK-zeta antibody (MBS1753738).Overlay histogram showing HL-60 cells stained with MBS1753738 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of HL-60 cells using anti-DGKZ/DGK-zeta antibody (MBS1753738).Overlay histogram showing HL-60 cells stained with MBS1753738 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DGKZ/DGK-zeta Antibody (MBS1753738, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Gross, M. B. Personal Communication. Baltimore, Md. 4/11/2014.
3. Topham, M. K., Bunting, M., Zimmerman, G. A., McIntyre, T. M., Blackshear, P. J., Prescott, S. M. Protein kinase C regulates the nuclear localization of diacylglycerol kinase-zeta. Nature 394: 697-700, 1998.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010]
Uniprot Description
DGKZ: Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity. Belongs to the eukaryotic diacylglycerol kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Kinase, lipid; Lipid Metabolism - glycerolipid; EC 2.7.1.107; Lipid Metabolism - glycerophospholipid
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: nucleoplasm; lamellipodium; cytoplasm; plasma membrane; nucleus
Molecular Function: enzyme inhibitor activity; protein C-terminus binding; lipid kinase activity; protein binding; metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity
Biological Process: G1 DNA damage checkpoint; platelet activation; cell migration; negative regulation of catalytic activity; protein kinase C activation; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; lipid phosphorylation; blood coagulation; phosphorylation
Research Articles on DGKZ
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Product Notes
The DGKZ dgkz (Catalog #AAA1753738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DGKZ/DGK-zeta Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DGKZ/DGK-zeta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| IHC-P: 2-5ug/ml|Human, Mouse| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the DGKZ dgkz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKZ/DGK-zeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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