Human EG-VEGF ELISA Kit | PROK1 elisa kit

Human EG-VEGF PicoKine ELISA Kit

Cat. Num. AAA175861

• Gene Names: PROK1; PK1; PRK1; EGVEGF
• Reactivity: Human
• Synonyms: EG-VEGF; Human EG-VEGF PicoKine ELISA Kit; Prokineticin-1; prokineticin 1; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PROK1; PROK1_HUMAN; PROK1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human EG-VEGF
• Sequence Length: 105

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 7pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human EG-VEGF PicoKine ELISA Kit standard curve )

Related Product Information for PROK1 elisa kit

Principle of the assay: human EG-VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EG-VEGF has been precoated onto 96-well plates. Standards(NSO, A20-F105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EG-VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EG-VEGF amount of sample captured in plate.
Background: EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands.

NCBI and Uniprot Product Information

• NCBI GI #: 17380189
• NCBI GeneID: 84432
• UniProt Accession #: P58294; Q5VWD4; Q8TC69
• Molecular Weight: 11,715 Da
• NCBI Official Full Name: Prokineticin-1
• NCBI Official Synonym Full Names: prokineticin 1
• NCBI Official Symbol: PROK1
• NCBI Official Synonym Symbols: PK1; PRK1; EGVEGF
• NCBI Protein Information: prokineticin-1; EG-VEGF; mambakine; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor
• UniProt Protein Name: Prokineticin-1
• Protein Family: Prokineticin
• UniProt Gene Name: PROK1
• UniProt Synonym Gene Names: EG-VEGF
• UniProt Entry Name: PROK1_HUMAN

NCBI Description

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]

Uniprot Description

PROK1: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Belongs to the AVIT (prokineticin) family.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular region

Molecular Function: growth factor activity

Biological Process: activation of MAPK activity; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; regulation of angiogenesis