Mouse E3 ubiquitin-protein ligase MARCH7 (MARCH7) ELISA Kit | MARCH7 elisa kit
Mouse E3 ubiquitin-protein ligase MARCH7 (MARCH7) ELISA Kit
Gene Names
March7; Axo; Axot; Gtrgeo17
Reactivity
Mouse
Synonyms
E3 ubiquitin-protein ligase MARCH7 (MARCH7); Mouse E3 ubiquitin-protein ligase MARCH7 (MARCH7) ELISA Kit; MARCH7; AXOT; DKFZp586F1122; MARCH-VII; RNF177; OTTHUMP00000204988; axotrophin; membrane-associated RING-CH protein VII; MARCH7 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse AXO. No significant cross-reactivity or interference between Mouse AXO and analogues was observed.
Sequence Length
693
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for MARCH7 elisa kit
This assay employs a two-site sandwich ELISA to quantitate AXO in samples. An antibody specific for AXO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AXO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AXO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AXO bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
76,599 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH7
NCBI Official Synonym Full Names
membrane-associated ring finger (C3HC4) 7
NCBI Official Symbol
March7
NCBI Official Synonym Symbols
Axo; Axot; Gtrgeo17
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH7
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH7
UniProt Gene Name
March7
UniProt Synonym Gene Names
Axot; MARCH-VII
Uniprot Description
E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in T-cell proliferation by regulating LIF secretion.
Research Articles on MARCH7
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Product Notes
The Mouse MARCH7 march7 (Catalog #AAA2800256) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2800256 ELISA Kit recognizes Mouse MARCH7. It is sometimes possible for the material contained within the vial of "E3 ubiquitin-protein ligase MARCH7 (MARCH7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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