Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA polymerase beta (POLB) Standard Curve.)
Mouse DNA Polymerase beta (POLB) ELISA Kit | POLB elisa kit
Mouse DNA Polymerase beta (POLB) ELISA Kit
Gene Names
Polb; A430088C08Rik
Reactivity
Mouse
Synonyms
DNA Polymerase beta (POLB); Mouse DNA Polymerase beta (POLB) ELISA Kit; POLB; MGC125976; DNA pol beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; POLB elisa kit
Reactivity
Mouse
Specificity
Mouse DNA polymerase beta (POLB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLB. No significant cross-reactivity or interference between Mouse POLB and analogues was observed.
Sequence Length
335
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA polymerase beta (POLB) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA polymerase beta (POLB) Standard Curve.)
Related Product Information for POLB elisa kit
Description: This Mouse DNA polymerase beta (POLB) ELISA Kit employs a two-site sandwich ELISA to quantitate POLB in samples. An antibody specific for POLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DNA polymerase beta maintains genome integrity by participating in base excision repair. Over expression of POLB mRNA has been correlated with a number of cancer types whereas deficiencies in POLB results in hypersensitivity to alkylating agents, induced apoptosis and chromosomal breaking therefore it is essential that POLB expression is tightly regulated. POLB gene is upregulated by CREB1 transcription factor binding to the cAMP response element(CRE) present in the promoter of the POLB gene in response to exposure to alkylating agents. POLB gene expression is also regulated at the post transcriptional level as the 3'UTR of the POLB mRNA has been shown to contain three stem-loop structures that influence gene expression.
Background: DNA polymerase beta maintains genome integrity by participating in base excision repair. Over expression of POLB mRNA has been correlated with a number of cancer types whereas deficiencies in POLB results in hypersensitivity to alkylating agents, induced apoptosis and chromosomal breaking therefore it is essential that POLB expression is tightly regulated. POLB gene is upregulated by CREB1 transcription factor binding to the cAMP response element(CRE) present in the promoter of the POLB gene in response to exposure to alkylating agents. POLB gene expression is also regulated at the post transcriptional level as the 3'UTR of the POLB mRNA has been shown to contain three stem-loop structures that influence gene expression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA polymerase beta
NCBI Official Synonym Full Names
polymerase (DNA directed), beta
NCBI Official Symbol
Polb
NCBI Official Synonym Symbols
A430088C08Rik
NCBI Protein Information
DNA polymerase beta
UniProt Protein Name
DNA polymerase beta
Protein Family
UniProt Gene Name
Polb
UniProt Entry Name
DPOLB_MOUSE
Research Articles on POLB
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Product Notes
The Mouse POLB polb (Catalog #AAA9717323) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717323 ELISA Kit recognizes Mouse POLB. It is sometimes possible for the material contained within the vial of "DNA Polymerase beta (POLB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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