Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) Standard Curve.)
Mouse DNA-Directed RNA Polymerases I And III Subunit RPAC2 (POLR1D) ELISA Kit | POLR1D elisa kit
Mouse DNA-Directed RNA Polymerases I And III Subunit RPAC2 (POLR1D) ELISA Kit
Gene Names
Polr1d; AC19; RPA16; Rpo1-3; 1110003G10Rik
Reactivity
Mouse
Synonyms
DNA-Directed RNA Polymerases I And III Subunit RPAC2 (POLR1D); Mouse DNA-Directed RNA Polymerases I And III Subunit RPAC2 (POLR1D) ELISA Kit; POLR1D; FLJ20616; MGC9850; POLR1C; RPA16; RPA9; RPAC2; RPO1-3; OTTHUMP00000018171; RNA polymerase I 16 kDa subunit; polymerase (RNA) I polypeptide D; POLR1D elisa kit
Reactivity
Mouse
Specificity
Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POLR1D. No significant cross-reactivity or interference between Mouse POLR1D and analogues was observed.
Sequence Length
133
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) Standard Curve.)
Related Product Information for POLR1D elisa kit
Description: This Mouse DNA-directed RNA polymerases I and III subunit RPAC2 (POLR1D) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR1D in samples. An antibody specific for POLR1D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POLR1D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR1D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR1D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The peak region of 13q contains POLR1D, a subunit of both RNA polymerases I and III. RNA polymerase I is involved in the production of 18S, 5.8S, and 28S rRNAs, while RNA polymerase III synthesizes small essential RNAs, such as tRNAs, 5S rRNA, and some snRNAs.There is not much information regarding this gene in the literature. POLR1D is overexpressed in 42% of the primary tumors, showing high correlation between expression and copy number. RPA16 cDNA encoding the 16-kDa subunit of mouse RNA polymerase I by a yeast two-hybrid system using mRPA40 as a bait. The deduced amino acid sequence shows 45% identity to the yeast subunit of RNA polymerases I and III, known to associate with AC40, and a local similarity to bacterial alpha subunit.
Background: The peak region of 13q contains POLR1D, a subunit of both RNA polymerases I and III. RNA polymerase I is involved in the production of 18S, 5.8S, and 28S rRNAs, while RNA polymerase III synthesizes small essential RNAs, such as tRNAs, 5S rRNA, and some snRNAs.There is not much information regarding this gene in the literature. POLR1D is overexpressed in 42% of the primary tumors, showing high correlation between expression and copy number. RPA16 cDNA encoding the 16-kDa subunit of mouse RNA polymerase I by a yeast two-hybrid system using mRPA40 as a bait. The deduced amino acid sequence shows 45% identity to the yeast subunit of RNA polymerases I and III, known to associate with AC40, and a local similarity to bacterial alpha subunit.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA-directed RNA polymerases I and III subunit RPAC2 isoform 1
NCBI Official Synonym Full Names
polymerase (RNA) I polypeptide D
NCBI Official Symbol
Polr1d
NCBI Official Synonym Symbols
AC19; RPA16; Rpo1-3; 1110003G10Rik
NCBI Protein Information
DNA-directed RNA polymerases I and III subunit RPAC2
UniProt Protein Name
DNA-directed RNA polymerases I and III subunit RPAC2
Protein Family
UniProt Gene Name
Polr1d
UniProt Synonym Gene Names
Rpo1-3; RNA polymerases I and III subunit AC2; RPA16
UniProt Entry Name
RPAC2_MOUSE
NCBI Description
This gene encodes an RNA polymerase subunit that is a component of both the RNA polymerase I and RNA polymerase III complexes. RNA polymerase I is associated with transcription of pre-ribosomal RNAs, while RNA polymerase III is associated with transcription of small RNAs. Pseudogenes of this gene have been defined on chromosomes 4 and 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]
Similar Products
Product Notes
The Mouse POLR1D polr1d (Catalog #AAA9718052) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718052 ELISA Kit recognizes Mouse POLR1D. It is sometimes possible for the material contained within the vial of "DNA-Directed RNA Polymerases I And III Subunit RPAC2 (POLR1D), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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