Mouse DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) ELISA Kit | DNMT1 elisa kit

Mouse DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) ELISA Kit

Cat. Num. AAA9718003

• Gene Names: Dnmt1; Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• Reactivity: Mouse
• Synonyms: DNA (Cytosine-5)-Methyltransferase 1 (DNMT1); Mouse DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) ELISA Kit; DNMT1; AIM; CXXC9; DNMT; FLJ16293; MCMT; MGC104992; CXXC finger protein 9; DNA methyltransferase 1; DNMT1 elisa kit

• Reactivity: Mouse
• Specificity: Mouse DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DNMT1. No significant cross-reactivity or interference between Mouse DNMT1 and analogues was observed.
• Sequence Length: 1620

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse DNA (cytosine-5)-methyltransferase 1 (DNMT1) Standard Curve.)

Related Product Information for DNMT1 elisa kit

Description: This Mouse DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DNMT1 in samples. An antibody specific for DNMT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNMT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNMT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNMT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: DNA (cytosine-5)-methyltransferases (DNMTs), such as DNMT1, maintain patterns of methylated cytosine residues in the mammalian genome. Genomic methylation patterns are reshaped during gametogenesis and early development and undergo programmed alterations during cellular differentiation. Methylation patterns are responsible for the repression of parasitic sequence elements and the expression status of genes subject to genomic imprinting and X inactivation. Faithful maintenance of methylation patterns is required for normal mammalian development, and aberrant methylation patterns are associated with certain human tumors and developmental abnormalities.A DNMT1 enzyme is thought to be responsible for most of the maintenance as well as the de novo methylation activities occurring in the somatic cells of vertebrates.

NCBI and Uniprot Product Information

• NCBI GI #: 1765919
• NCBI GeneID: 13433
• UniProt Accession #: P13864
• NCBI Official Full Name: DNA methyltransferase 1
• NCBI Official Synonym Full Names: DNA methyltransferase (cytosine-5) 1
• NCBI Official Symbol: Dnmt1
• NCBI Official Synonym Symbols: Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: Dnmt1
• UniProt Synonym Gene Names: Dnmt; Met1; Uim; Dnmt1; Met-1; DNA MTase MmuI; M.MmuI
• UniProt Entry Name: DNMT1_MOUSE

NCBI Description

This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]