Testing Data
(Staining of porcine peripheral blood platelets with Mouse anti Porcine CD61:FITC)
Mouse CD61 Monoclonal Antibody | anti-CD61 antibody
MOUSE ANTI PIG CD61:FITC
Gene Names
ITGB3; CD61
Reactivity
Reacts with: Dog, Human, Bovine, Horse; N.B. Antibody reactivity and working conditions may vary between species.
Applications
Flow Cytometry, Functional Assay
Synonyms
CD61; Monoclonal Antibody; MOUSE ANTI PIG CD61:FITC; anti-CD61 antibody
Host
Mouse
Reactivity
Reacts with: Dog, Human, Bovine, Horse; N.B. Antibody reactivity and working conditions may vary between species.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
JM2E5
Form/Format
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
784
Applicable Applications for anti-CD61 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry:
Suggested Dilution: Neat - 1/10
Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Suggested Dilution: Neat - 1/10
Use 10 ul of the suggested working dilution to label 106 cells in 100ul.
Target Species
Pig
Max Ex/Em
Fluorophore: FITC
Excitation Max (nm): 490
Emission Max (nm): 525
Excitation Max (nm): 490
Emission Max (nm): 525
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Immunogen
Porcine peripheral blood mononuclear cells.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 - Ag14 myeloma cell line.
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of porcine peripheral blood platelets with Mouse anti Porcine CD61:FITC)
Testing Data
(Staining of porcine peripheral blood platelets with Mouse anti Porcine CD61:FITC)
Related Product Information for anti-CD61 antibody
Mouse anti Pig CD61 antibody, clone JM2E5 recognizes the porcine CD61 cell surface antigen, also known as platelet glycoprotein IIIa.
CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells (Moreno et al. 2002). CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney (Piriou-Guzylack et al., 2008), spleen, intestinal mucosa and Leydig cells in testis (Moreno et al. 2002).
The epitope recognized by this antibody is not sensitive to EDTA.
Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting.
CD61 is present on the megakaryocyte/platelet lineage, granulocytes, cells from the monocyte/macrophage lineage and endothelial cells (Moreno et al. 2002). CD61 is also broadly expressed on tissues, such as epithelial cells from tubules in the kidney (Piriou-Guzylack et al., 2008), spleen, intestinal mucosa and Leydig cells in testis (Moreno et al. 2002).
The epitope recognized by this antibody is not sensitive to EDTA.
Mouse anti Pig CD61 antibody, clone JM2E5 detects a band of approximately 85 kDa in porcine platelet lysates by western blotting.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
integrin beta-3
NCBI Official Symbol
ITGB3
NCBI Official Synonym Symbols
CD61
NCBI Protein Information
integrin beta-3; beta3 integrin; glycoprotein GPIIIa; integrin beta 3
Research Articles on CD61
Similar Products
Product Notes
The CD61 (Catalog #AAA215733) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI PIG CD61:FITC reacts with Reacts with: Dog, Human, Bovine, Horse; N.B. Antibody reactivity and working conditions may vary between species. and may cross-react with other species as described in the data sheet. AAA Biotech's CD61 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Suggested Dilution: Neat - 1/10 Use 10 ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD61 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD61, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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