Human Dipeptidyl peptidase 9 ELISA Kit | DPP9 elisa kit

Human Dipeptidyl peptidase 9 ELISA Kit

Cat. Num. AAA763178

• Gene Names: DPP9; DP9; DPLP9; DPRP2; DPRP-2
• Reactivity: Human
• Synonyms: Dipeptidyl peptidase 9; Human Dipeptidyl peptidase 9 ELISA Kit; DPP9/DPLP9/DPRP2/DP9/DPP IX/DPRP-2/dipeptidyl peptidase 9/Dipeptidyl peptidase IV-related protein 2/Dipeptidyl peptidase IX/Dipeptidyl peptidase-like protein 9/dipeptidylpeptidase 9/dipeptidyl-peptidase 9; DPP9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DPP9. No significant cross-reactivity or interference between DPP9 and analogues was observed.
• Sequence Length: 892

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPP9 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 194394146
• NCBI GeneID: 91039
• NCBI Accession #: NP_631898.3
• NCBI GenBank Nucleotide #: NM_139159.4
• Molecular Weight: 95,013 Da
• NCBI Official Full Name: dipeptidyl peptidase 9
• NCBI Official Synonym Full Names: dipeptidyl peptidase 9
• NCBI Official Symbol: DPP9
• NCBI Official Synonym Symbols: DP9; DPLP9; DPRP2; DPRP-2
• NCBI Protein Information: dipeptidyl peptidase 9
• UniProt Protein Name: Dipeptidyl peptidase 9
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: DPP9
• UniProt Synonym Gene Names: DPRP2; DP9; DPRP-2; DPP IX; DPLP9
• UniProt Entry Name: DPP9_HUMAN

NCBI Description

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

DPP9: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Belongs to the peptidase S9B family. DPPIV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.14.5; Protease

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: aminopeptidase activity; identical protein binding; serine-type peptidase activity

Biological Process: proteolysis