MCP-4 Recombinant Protein | CCL13 recombinant protein

MCP-4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120297

• Gene Names: CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MCP-4; MCP-4 Protein; Human; Recombinant (His Tag); Human CCL13/MCP-4 Protein (His Tag); CCL13 Protein; CKb10 Protein; MCP4 Protein; NCC-1 Protein; NCC1 Protein; SCYA13 Protein; SCYL1 Protein; chemokine (C-C motif) ligand 13; CCL13 recombinant protein

• Host: Yeast
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln24-Thr98

• Species: Human
• Predicted N Terminal: Gln 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding human CCL13 (NP_005399.1)(Gln24-Thr98) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL13 recombinant protein

Background: Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is a member of a distinct, structurally-related subclass of CC chemokines mainly involved in recruitment of eosinphils to inflammatory sites. CCL13/MCP-4, is a CC family chemokine that is chemoattractant for eosinophils, basophils, monocytes, macrophages, immature dendritic cells, and T cells, and its capable of inducing crucial immuno-modulatory responses through its effects on epithelial, muscular and endothelial cells. Similar to other CC chemokines, CCL13 binds to several chemokine receptors (CCR1, CCR2 and CCR3), allowing it to elicit different effects on its target cells. A number of studies have shown that CCL13 is involved in many chronic inflammatory diseases, in which it functions as a pivotal molecule involved in the selective recruitment of cell lineages to the inflamed tissues and their subsequent activation. MCP-4/CCL13 is secreted from chondrocytes and activates the proliferation of rheumatoid synovial cells, thereby leading to joint destruction in RA. The interferon-gamma in combination with interleukin-1beta/tumor necrosis factor-alpha activates the production of MCP-4/CCL13 from chondrocytes in RA joints, and that secreted MCP-4/CCL13 enhances fibroblast-like synoviocyte proliferation by activating the extracellular signal-regulated kinase mitogen-activated protein kinase cascade. CCL13 may have some role in the pathogenesis of systemic sclerosis (SSc).

NCBI and Uniprot Product Information

• NCBI GI #: 4885587
• NCBI GeneID: 6357
• NCBI Accession #: NP_005399.1
• NCBI GenBank Nucleotide #: NM_005408.2
• UniProt Accession #: Q99616; O95689; Q6ICQ6
• Molecular Weight: 10,986 Da
• NCBI Official Full Name: C-C motif chemokine 13
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 13
• NCBI Official Symbol: CCL13
• NCBI Official Synonym Symbols: NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• NCBI Protein Information: C-C motif chemokine 13; CK-beta-10; monocyte chemoattractant protein 4; monocyte chemotactic protein 4; new CC chemokine 1; small inducible cytokine subfamily A (Cys-Cys), member 13; small-inducible cytokine A13
• UniProt Protein Name: C-C motif chemokine 13
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL13
• UniProt Synonym Gene Names: MCP4; NCC1; SCYA13; MCP-4
• UniProt Entry Name: CCL13_HUMAN

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: chemokine activity; receptor binding

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; cytoskeleton organization and biogenesis; immune response; signal transduction; inflammatory response; chemotaxis