Human E3 Ubiquitin-Protein Ligase Synoviolin (SYVN1) ELISA Kit | SYVN1 elisa kit

Human E3 Ubiquitin-Protein Ligase Synoviolin (SYVN1) ELISA Kit

Cat. Num. AAA9714112

• Gene Names: SYVN1; DER3; HRD1
• Reactivity: Human
• Synonyms: E3 Ubiquitin-Protein Ligase Synoviolin (SYVN1); Human E3 Ubiquitin-Protein Ligase Synoviolin (SYVN1) ELISA Kit; SYVN1; HRD1; KIAA1810; MGC40372; synoviolin 1; SYVN1 elisa kit

• Reactivity: Human
• Specificity: Human E3 ubiquitin-protein ligase synoviolin (SYVN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYVN1. No significant cross-reactivity or interference between Human SYVN1 and analogues was observed.
• Sequence Length: 617

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human E3 ubiquitin-protein ligase synoviolin (SYVN1) Standard Curve.)

Related Product Information for SYVN1 elisa kit

Description: This Human E3 ubiquitin-protein ligase synoviolin (SYVN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYVN1 in samples. An antibody specific for SYVN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYVN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYVN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYVN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: E3 ubiquitin-protein ligase synoviolin (SYVN1) involved in endoplasmic reticulum (ER)-associated degradation. E3 ubiquitin-protein ligase synoviolin removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. E3 ubiquitin-protein ligase synoviolin also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 27436927
• NCBI GeneID: 84447
• NCBI Accession #: NP_757385.1
• NCBI GenBank Nucleotide #: NP_757385.1
• UniProt Accession #: Q86TM6
• NCBI Official Full Name: E3 ubiquitin-protein ligase synoviolin isoform b
• NCBI Official Synonym Full Names: synoviolin 1
• NCBI Official Symbol: SYVN1
• NCBI Official Synonym Symbols: DER3; HRD1
• NCBI Protein Information: E3 ubiquitin-protein ligase synoviolin
• UniProt Protein Name: E3 ubiquitin-protein ligase synoviolin
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: SYVN1
• UniProt Synonym Gene Names: HRD1; KIAA1810

NCBI Description

This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]

Uniprot Description

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:26471130). Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:12459480, PubMed:12646171, PubMed:12975321, PubMed:14593114, PubMed:16289116, PubMed:16847254, PubMed:17059562, PubMed:17141218, PubMed:17170702, PubMed:22607976, PubMed:26471130). Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation (PubMed:17141218). Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis (PubMed:17170702). Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 ().