Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dentin matrix acidic phosphoprotein 1 (DMP1) Standard Curve.)
Mouse Dentin Matrix Acidic Phosphoprotein 1 (DMP1) ELISA Kit | DMP1 elisa kit
Mouse Dentin Matrix Acidic Phosphoprotein 1 (DMP1) ELISA Kit
Gene Names
Dmp1; PP; AG1; Dmp; DMP-1; AV020965
Reactivity
Mouse
Synonyms
Dentin Matrix Acidic Phosphoprotein 1 (DMP1); Mouse Dentin Matrix Acidic Phosphoprotein 1 (DMP1) ELISA Kit; DMP1; ARHP; ARHR; DMP-1; dentin matrix protein 1; DMP1 elisa kit
Reactivity
Mouse
Specificity
Mouse Dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMP1. No significant cross-reactivity or interference between Mouse DMP1 and analogues was observed.
Sequence Length
503
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dentin matrix acidic phosphoprotein 1 (DMP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Dentin matrix acidic phosphoprotein 1 (DMP1) Standard Curve.)
Related Product Information for DMP1 elisa kit
Description: This Mouse Dentin matrix acidic phosphoprotein 1 (DMP1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMP1 in samples. An antibody specific for DMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DMP1 is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.
Background: DMP1 is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Dentin matrix acidic phosphoprotein 1
NCBI Official Synonym Full Names
dentin matrix protein 1
NCBI Official Symbol
Dmp1
NCBI Official Synonym Symbols
PP; AG1; Dmp; DMP-1; AV020965
NCBI Protein Information
dentin matrix acidic phosphoprotein 1
UniProt Protein Name
Dentin matrix acidic phosphoprotein 1
Protein Family
UniProt Gene Name
Dmp1
UniProt Synonym Gene Names
Dmp; DMP-1; Dentin matrix protein 1
UniProt Entry Name
DMP1_MOUSE
Uniprot Description
DMP1: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. Defects in DMP1 are the cause of rickets hypophosphatemic autosomal recessive type 1 (ARHR1). A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: nucleoplasm; proteinaceous extracellular matrix; intracellular membrane-bound organelle; cytoplasm; nucleolus; extracellular region; nucleus
Molecular Function: extracellular matrix binding
Biological Process: extracellular matrix organization and biogenesis; ossification; biomineral formation
Research Articles on DMP1
Similar Products
Product Notes
The Mouse DMP1 dmp1 (Catalog #AAA9717851) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717851 ELISA Kit recognizes Mouse DMP1. It is sometimes possible for the material contained within the vial of "Dentin Matrix Acidic Phosphoprotein 1 (DMP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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