Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delta (24)-sterol reductase (DHCR24) Standard Curve.)
Mouse Delta (24)-Sterol Reductase (DHCR24) ELISA Kit | DHCR24 elisa kit
Mouse Delta (24)-Sterol Reductase (DHCR24) ELISA Kit
Gene Names
Dhcr24; mKIAA0018; 2310076D10Rik; 5830417J06Rik
Reactivity
Mouse
Synonyms
Delta (24)-Sterol Reductase (DHCR24); Mouse Delta (24)-Sterol Reductase (DHCR24) ELISA Kit; DHCR24; KIAA0018; Nbla03646; SELADIN1; seladin-1; 3 beta-hydroxysterol delta 24-reductase; diminuto/dwarf1 homolog; putative protein product of Nbla03646; selective AD indicator 1; DHCR24 elisa kit
Reactivity
Mouse
Specificity
Mouse Delta (24)-sterol reductase (DHCR24) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DHCR24. No significant cross-reactivity or interference between Mouse DHCR24 and analogues was observed.
Sequence Length
516
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delta (24)-sterol reductase (DHCR24) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delta (24)-sterol reductase (DHCR24) Standard Curve.)
Related Product Information for DHCR24 elisa kit
Description: This Mouse Delta (24)-sterol reductase (DHCR24) ELISA Kit employs a two-site sandwich ELISA to quantitate DHCR24 in samples. An antibody specific for DHCR24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DHCR24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR24 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 have been associated with desmosterolosis. Also, reduced expression of DHCR24 occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
Background: DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 have been associated with desmosterolosis. Also, reduced expression of DHCR24 occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
delta(24)-sterol reductase
NCBI Official Synonym Full Names
24-dehydrocholesterol reductase
NCBI Official Symbol
Dhcr24
NCBI Official Synonym Symbols
mKIAA0018; 2310076D10Rik; 5830417J06Rik
NCBI Protein Information
delta(24)-sterol reductase
UniProt Protein Name
Delta(24)-sterol reductase
Protein Family
UniProt Gene Name
Dhcr24
UniProt Synonym Gene Names
Kiaa0018
UniProt Entry Name
DHC24_MOUSE
Research Articles on DHCR24
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Product Notes
The Mouse DHCR24 dhcr24 (Catalog #AAA9717904) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717904 ELISA Kit recognizes Mouse DHCR24. It is sometimes possible for the material contained within the vial of "Delta (24)-Sterol Reductase (DHCR24), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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