Rabbit anti-Human DHCR24 Polyclonal Antibody | anti-DHCR24 antibody

DHCR24 (Delta(24)-sterol Reductase, 24-dehydrocholesterol Reductase, 3-beta-hydroxysterol delta-24-reductase, Diminuto/Dwarf1 Homolog, Seladin-1, KIAA0018)

Cat. Num. AAA6008974

• Gene Names: DHCR24; DCE; SELADIN1; Nbla03646; seladin-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DHCR24; Polyclonal Antibody; DHCR24 (Delta(24)-sterol Reductase; 24-dehydrocholesterol Reductase; 3-beta-hydroxysterol delta-24-reductase; Diminuto/Dwarf1 Homolog; Seladin-1; KIAA0018); Anti -DHCR24 (Delta(24)-sterol Reductase; anti-DHCR24 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DHCR24.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH

• Applicable Applications for anti-DHCR24 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human DHCR24, aa1-516 (NP_055577.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DHCR24 expression in transfected 293T cell line by DHCR24 polyclonal antibody. Lane 1: DHCR24 transfected lysate (60.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DHCR24 antibody

DHCR24 is a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. This protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of its gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Product Categories/Family for anti-DHCR24 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13375618
• NCBI GeneID: 1718
• NCBI Accession #: NP_055577.1
• NCBI GenBank Nucleotide #: NM_014762.3
• UniProt Accession #: Q15392; Q9HBA8; D3DQ51
• Molecular Weight: 60,101 Da
• NCBI Official Full Name: delta(24)-sterol reductase
• NCBI Official Synonym Full Names: 24-dehydrocholesterol reductase
• NCBI Official Symbol: DHCR24
• NCBI Official Synonym Symbols: DCE; SELADIN1; Nbla03646; seladin-1
• NCBI Protein Information: delta(24)-sterol reductase; delta(24)-sterol reductase; seladin 1; diminuto/dwarf1 homolog; selective AD indicator 1; desmosterol-to-cholesterol enzyme; 3 beta-hydroxysterol delta 24-reductase; 3-beta-hydroxysterol delta-24-reductase
• UniProt Protein Name: Delta(24)-sterol reductase
• Protein Family: Delta(24)-sterol reductase
• UniProt Gene Name: DHCR24
• UniProt Synonym Gene Names: KIAA0018
• UniProt Entry Name: DHC24_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.

Protein type: Lipid Metabolism - steroid biosynthesis; EC 1.3.1.72; Oxidoreductase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cytoskeleton; membrane; endoplasmic reticulum; integral to membrane; nucleus; cytosol

Molecular Function: delta24(24-1) sterol reductase activity; UDP-N-acetylmuramate dehydrogenase activity; enzyme binding; peptide antigen binding; FAD binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; delta24-sterol reductase activity

Biological Process: skin development; tissue development; apoptosis; response to hormone stimulus; negative regulation of caspase activity; cholesterol biosynthetic process; negative regulation of cell proliferation; amyloid precursor protein catabolic process; protein localization; Ras protein signal transduction; male genitalia development; response to oxidative stress; cell cycle arrest; plasminogen activation; negative regulation of apoptosis

Disease: Desmosterolosis