Typical Testing Data/Standard Curve (for reference only)
Mouse Cytochrome P450 2C8 (CYP2C8) ELISA Kit | CYP2C8 elisa kit
Mouse Cytochrome P450 2C8 (CYP2C8) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CYP2C8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CYP2C8 antibody and an CYP2C8-HRP conjugate. The assay sample and buffer are incubated together with CYP2C8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP2C8 concentration since CYP2C8 from samples and CYP2C8-HRP conjugate compete for the anti-CYP2C8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP2C8 from the sample, fewer sites are left to bind CYP2C8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP2C8 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Uniprot Description
CYP2C8: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it generates only 14,15- and 11,12-cis-epoxyeicosatrienoic acids. It is the principal enzyme responsible for the metabolism the anti- cancer drug paclitaxel (taxol). Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Oxidoreductase; EC 1.14.14.1
Chromosomal Location of Human Ortholog: 10q23.33
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm
Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding; monooxygenase activity
Biological Process: organic acid metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; epoxygenase P450 pathway; drug metabolic process
Research Articles on CYP2C8
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Product Notes
The Mouse CYP2C8 cyp2c8 (Catalog #AAA7222138) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7222138 ELISA Kit recognizes Mouse CYP2C8. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2C8 (CYP2C8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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