Mouse Crystallin Beta A1 (CRYbA1) ELISA Kit | CRYbA1 elisa kit

Mouse Crystallin Beta A1 (CRYbA1) ELISA Kit

Cat. Num. AAA2705771

• Gene Names: CRYBA1; CRYB1; CTRCT10
• Reactivity: Mouse
• Synonyms: Crystallin Beta A1 (CRYbA1); Mouse Crystallin Beta A1 (CRYbA1) ELISA Kit; CRYB1; Eye Lens Structural Protein; CRYbA1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Crystallin Beta A1 (CRYbA1).; No significant cross-reactivity or interference between Crystallin Beta A1 (CRYbA1) and analogues was observed.
• Sequence Length: 198

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Crystallin Beta A1 (CRYbA1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Crystallin Beta A1 (CRYbA1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRYbA1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Beta A1 (CRYbA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crystallin Beta A1 (CRYbA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Beta A1 (CRYbA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crystallin Beta A1 (CRYbA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 109730599
• NCBI GeneID: 1411
• Molecular Weight: 23,191 Da
• NCBI Official Full Name: Crystallin, beta A1
• NCBI Official Synonym Full Names: crystallin beta A1
• NCBI Official Symbol: CRYBA1
• NCBI Official Synonym Symbols: CRYB1; CTRCT10
• NCBI Protein Information: beta-crystallin A3
• UniProt Protein Name: Beta-crystallin A3
• Protein Family: Beta-crystallin
• UniProt Gene Name: CRYBA1
• UniProt Synonym Gene Names: CRYB1

NCBI Description

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'. [provided by RefSeq, Jul 2008]

Uniprot Description

Crystallins are the dominant structural components of the vertebrate eye lens.