Typical Testing Data/Standard Curve (for reference only)
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Mouse cocaine- and amphetamine-regulated transcript (non-HGNC gene) (CART) ELISA Kit | CART elisa kit
Mouse cocaine- and amphetamine-regulated transcript (non-HGNC gene) (CART) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: CART ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CART antibody and an CART-HRP conjugate. The assay sample and buffer are incubated together with CART-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CART concentration since CART from samples and CART-HRP conjugate compete for the anti-CART antibody binding site. Since the number of sites is limited, as more sites are occupied by CART from the sample, fewer sites are left to bind CART-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CART concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted protein which is processed by prohormone/proprotein convertases to produce smaller, biologically active peptides. Expression of the transcript for this gene is regulated by certain drugs such as cocaine, and the encoded protein is thought to be involved in the regulation of appetite and stress. Mutations in this gene are associated with susceptibility to obesity.[provided by RefSeq, Nov 2009]
Uniprot Description
CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Hormone
Chromosomal Location of Human Ortholog: 5q13.2
Cellular Component: extracellular space; secretory granule
Molecular Function: protein binding
Biological Process: negative regulation of osteoclast differentiation; activation of MAPKK activity; adult feeding behavior; cellular response to starvation; signal transduction; negative regulation of appetite; G-protein coupled receptor protein signaling pathway; synaptic transmission; cell-cell signaling; neuropeptide signaling pathway; positive regulation of epinephrine secretion; cell glucose homeostasis; circadian regulation of gene expression; negative regulation of bone resorption; regulation of insulin secretion; positive regulation of blood pressure; positive regulation of transmission of nerve impulse
Disease: Obesity
Research Articles on CART
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Product Notes
The Mouse CART cartpt (Catalog #AAA7252301) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7252301 ELISA Kit recognizes Mouse CART. It is sometimes possible for the material contained within the vial of "cocaine- and amphetamine-regulated transcript (non-HGNC gene) (CART), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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