Mouse CMRF35-like molecule 8 (CD300A) ELISA Kit | CD300A elisa kit

Mouse CMRF35-like molecule 8 (CD300A) ELISA Kit

Cat. Num. AAA7221809

• Gene Names: CD300A; IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9
• Reactivity: Mouse
• Synonyms: CMRF35-like molecule 8 (CD300A); Mouse CMRF35-like molecule 8 (CD300A) ELISA Kit; CD300A elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CD300A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD300A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD300A elisa kit

Principle of the assay: CD300A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD300A antibody and an CD300A-HRP conjugate. The assay sample and buffer are incubated together with CD300A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD300A concentration since CD300A from samples and CD300A-HRP conjugate compete for the anti-CD300A antibody binding site. Since the number of sites is limited, as more sites are occupied by CD300A from the sample, fewer sites are left to bind CD300A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD300A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD300A elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 56550061
• NCBI GeneID: 11314
• NCBI Accession #: NP_009192.2
• NCBI GenBank Nucleotide #: NM_007261.3
• Molecular Weight: 16,517 Da
• NCBI Official Full Name: CMRF35-like molecule 8 isoform 1
• NCBI Official Synonym Full Names: CD300a molecule
• NCBI Official Symbol: CD300A
• NCBI Official Synonym Symbols: IRC1; IRC2; CLM-8; IRp60; IGSF12; CMRF35H; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9
• NCBI Protein Information: CMRF35-like molecule 8; CD300a antigen; NK inhibitory receptor; leukocyte membrane antigen; inhibitory receptor protein 60; CD300 antigen-like family member A; immunoglobulin superfamily member 12; CMRF35H leukocyte immunoglobulin-like receptor
• UniProt Protein Name: CMRF35-like molecule 8
• Protein Family: CMRF35-like molecule
• UniProt Gene Name: CD300A
• UniProt Synonym Gene Names: CMRF35H; IGSF12; HSPC083; CLM-8; CMRF35-H9; IgSF12; IRp60
• UniProt Entry Name: CLM8_HUMAN

NCBI Description

This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MAIR-I: Inhibitory receptor which may contribute to the down- regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Belongs to the CD300 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; phosphatidylethanolamine binding; phosphatidylserine binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of MAP kinase activity; negative regulation of NK T cell activation; negative regulation of phagocytosis, engulfment; negative regulation of fibroblast proliferation; immune system process; negative regulation of B cell receptor signaling pathway; regulation of T cell receptor signaling pathway; negative regulation of mast cell degranulation; negative regulation of mast cell activation during immune response; negative regulation of B cell proliferation; signal transduction; cell adhesion