Typical Testing Data/Standard Curve (for reference only)
Mouse CMP-sialic acid transporter (SLC35A1) ELISA Kit | SLC35A1 elisa kit
Mouse CMP-sialic acid transporter (SLC35A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SLC35A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SLC35A1 antibody and an SLC35A1-HRP conjugate. The assay sample and buffer are incubated together with SLC35A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC35A1 concentration since SLC35A1 from samples and SLC35A1-HRP conjugate compete for the anti-SLC35A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC35A1 from the sample, fewer sites are left to bind SLC35A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC35A1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is found in the membrane of the Golgi apparatus, where it transports nucleotide sugars into the Golgi. One such nucleotide sugar is CMP-sialic acid, which is imported into the Golgi by the encoded protein and subsequently glycosylated. Defects in this gene are a cause of congenital disorder of glycosylation type 2F (CDG2F). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]
Uniprot Description
SLC35A1: Transports CMP-sialic acid from the cytosol into Golgi vesicles where glycosyltransferases function. Defects in SLC35A1 are the cause of congenital disorder of glycosylation type 2F (CDG2F). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the nucleotide-sugar transporter family. SLC35A subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q15
Cellular Component: Golgi membrane; Golgi apparatus; integral to plasma membrane
Molecular Function: CMP-sialic acid transmembrane transporter activity; sugar:hydrogen ion symporter activity
Biological Process: proton transport; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate transport; carbohydrate metabolic process; protein modification process; protein amino acid N-linked glycosylation via asparagine; CMP-sialic acid transport; post-translational protein modification; transmembrane transport
Disease: Congenital Disorder Of Glycosylation, Type Iif
Research Articles on SLC35A1
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Product Notes
The Mouse SLC35A1 slc35a1 (Catalog #AAA7201828) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7201828 ELISA Kit recognizes Mouse SLC35A1. It is sometimes possible for the material contained within the vial of "CMP-sialic acid transporter (SLC35A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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