Western Blot (WB)
(Western blot analysis of AGR2 in Human Duodenum lysate (35μg protein in RIPA buffer) using AGR2 antibody (0.01μg/ml).)
Goat AGR2 Polyclonal Antibody | anti-AGR2 antibody
AGR2 (Anterior Gradient 2 Homolog (Xenopus laevis), AG2, GOB-4, HAG-2, XAG-2, Secreted Cement Gland Homolog)
Gene Names
AGR2; AG2; GOB-4; HAG-2; XAG-2; PDIA17
Reactivity
Canine, Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
AGR2; Polyclonal Antibody; AGR2 (Anterior Gradient 2 Homolog (Xenopus laevis); AG2; GOB-4; HAG-2; XAG-2; Secreted Cement Gland Homolog); Anti -AGR2 (Anterior Gradient 2 Homolog (Xenopus laevis); anti-AGR2 antibody
Host
Goat
Reactivity
Canine, Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes human AGR2 at ~17-18kD in Human Colon, Duodenum and Lung lysates (calculated MW of 20.0kD according to NP_006399.1). Species Crossreactivity: Canine and mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.5 mg/ml tris-saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Applicable Applications for anti-AGR2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:128,000
Western Blot: 0.01 g/ml
Dilution: ELISA: 1:128,000
Western Blot: 0.01 g/ml
Immunogen
Synthetic peptide corresponding to human AGR2.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of AGR2 in Human Duodenum lysate (35μg protein in RIPA buffer) using AGR2 antibody (0.01μg/ml).)
Western Blot (WB)
(Western blot analysis of AGR2 in Human Duodenum lysate (35μg protein in RIPA buffer) using AGR2 antibody (0.01μg/ml).)
Related Product Information for anti-AGR2 antibody
The anterior gradient protein-2 (AGR2) is a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. It has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells. Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells (1). Specifically, AGR2 may serve as a useful molecular marker and/or potential therapeutic target for hormone-responsive breast tumors (2). AGR2 expression promotes tumor growth in esophageal adenocarcinoma cells as well. Expression was detected in proliferating and differentiated intestinal cells of secretory lineage, suggesting that AGR2 may be important for the growth and development of the intestine as well as esophageal adenocarcinomas (3).
Product Categories/Family for anti-AGR2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,979 Da
NCBI Official Full Name
anterior gradient protein 2 homolog
NCBI Official Synonym Full Names
anterior gradient homolog 2 (Xenopus laevis)
NCBI Official Symbol
AGR2
NCBI Official Synonym Symbols
AG2; GOB-4; HAG-2; XAG-2; PDIA17
NCBI Protein Information
anterior gradient protein 2 homolog; AG-2; HPC8; OTTHUMP00000116014; OTTHUMP00000201755; anterior gradient 2 homolog; secreted cement gland homolog; secreted cement gland protein XAG-2 homolog; protein disulfide isomerase family A, member 17
UniProt Protein Name
Anterior gradient protein 2 homolog
Protein Family
UniProt Gene Name
AGR2
UniProt Synonym Gene Names
AG2
UniProt Entry Name
AGR2_HUMAN
Uniprot Description
AGR2: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked. Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. Belongs to the AGR family.
Protein type: Oncoprotein; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7p21.3
Cellular Component: mitochondrion; endoplasmic reticulum; extracellular region
Molecular Function: protein binding
Research Articles on AGR2
Similar Products
Product Notes
The AGR2 agr2 (Catalog #AAA620554) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The AGR2 (Anterior Gradient 2 Homolog (Xenopus laevis), AG2, GOB-4, HAG-2, XAG-2, Secreted Cement Gland Homolog) reacts with Canine, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AGR2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:128,000 Western Blot: 0.01 g/ml. Researchers should empirically determine the suitability of the AGR2 agr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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