Typical Standard Curve/Testing Data
Mouse Choline-phosphate cytidylyltransferase A (PCYT1A) ELISA Kit | PCYT1A elisa kit
Mouse Choline-phosphate cytidylyltransferase A (PCYT1A) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: PCYT1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCYT1A antibody and an PCYT1A-HRP conjugate. The assay sample and buffer are incubated together with PCYT1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCYT1A concentration since PCYT1A from samples and PCYT1A-HRP conjugate compete for the anti-PCYT1A antibody binding site. Since the number of sites is limited, as more sites are occupied by PCYT1A from the sample, fewer sites are left to bind PCYT1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCYT1A concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]
Uniprot Description
PCYT1A: Controls phosphatidylcholine synthesis. Homodimer. By phosphorylation. Belongs to the cytidylyltransferase family.
Protein type: Lipid Metabolism - glycerophospholipid; EC 2.7.7.15; Transferase
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: endoplasmic reticulum membrane; glycogen granule; cytosol
Molecular Function: choline-phosphate cytidylyltransferase activity; protein homodimerization activity; lipid binding
Biological Process: CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process
Disease: Spondylometaphyseal Dysplasia With Cone-rod Dystrophy
Research Articles on PCYT1A
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Product Notes
The Mouse PCYT1A pcyt1a (Catalog #AAA7214406) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214406 ELISA Kit recognizes Mouse PCYT1A. It is sometimes possible for the material contained within the vial of "Choline-phosphate cytidylyltransferase A (PCYT1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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